10.1371/journal.pone.0070272.g001
Kirstin Hobiger
Kirstin
Hobiger
Tillmann Utesch
Tillmann
Utesch
Maria Andrea Mroginski
Maria Andrea
Mroginski
Guiscard Seebohm
Guiscard
Seebohm
Thomas Friedrich
Thomas
Friedrich
Topology scheme of Ci-VSP.
Public Library of Science
2013
Anatomy and physiology
electrophysiology
biophysics
Biophysics simulations
Computational biology
Macromolecular structure analysis
protein structure
Molecular cell biology
Signal transduction
Signaling cascades
Phospholipid signaling cascade
Polyphosphoinositide signaling cascade
Signaling in cellular processes
Lipid signaling
Transmembrane signaling
Signaling in selected disciplines
Neurological signaling
Oncogenic signaling
Signaling pathways
Phosphoinositide signal transduction
Mechanisms of signal transduction
neuroscience
Cellular neuroscience
ion channels
2013-07-29 07:32:35
Figure
https://plos.figshare.com/articles/figure/_Topology_scheme_of_Ci_VSP_/759321
<p><i>(</i><b><i>A</i></b><i>)</i> Topology scheme of Ci-VSP with the transmembrane voltage sensor (VSD) and the intracellular catalytic domain (CD), which comprises the phosphatase (PD) and the C2-domain. VSD and CD are connected via the linker sequence M240–K257. <i>(</i><b><i>B</i></b><i>)</i> Three-dimensional structure of the Ci-VSP CD based on the crystal structure by Liu et al. (PDB entry 3V0H) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070272#pone.0070272-Liu1" target="_blank">[14]</a>. The P- (orange), the TI- (blue), and the WPD loop (yellow) form the active site of the CD. The linker (colored in red, with R253 shown as sticks) is oriented toward the TI loop, as proposed by Liu et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070272#pone.0070272-Liu1" target="_blank">[14]</a>. <i>(</i><b><i>C</i></b><i>)</i> Amino acid alignment of Ci-VSP’s linker (M240–K257), active site (H362–R369), and the TI loop (R398–S414) with regions of the homolog PTEN (Hs, Homo sapiens). Amino acid identities are highlighted with a gray background. Putative interacting partners in the linker and the TI loop are denoted with blue and red asterisks, respectively. Single and multiple altered Ci-VSP mutants are aligned as well (in red letters: mutated positions). In the single neutralization mutants, the X stands for N (in case aspartate was the native residue) or for Q (in case of glutamate).</p>