Inhibitory activity of tenofovir, adefovir, and PMEO-DAPym against HSV-2 in primary M/M cultures as determined with the CPE reduction assay and the PFU reduction assay.
Jan Balzarini
Graciela Andrei
Emanuela Balestra
Dana Huskens
Christophe Vanpouille
Andrea Introini
Sonia Zicari
Sandra Liekens
Robert Snoeck
Antonín Holý
Carlo-Federico Perno
Leonid Margolis
Dominique Schols
10.1371/journal.ppat.1003456.t003
https://plos.figshare.com/articles/dataset/_Inhibitory_activity_of_tenofovir_adefovir_and_PMEO_DAPym_against_HSV_2_in_primary_M_M_cultures_as_determined_with_the_CPE_reduction_assay_and_the_PFU_reduction_assay_/755055
<p>Virus production was evaluated on day 6 post infection. Infectious HSV-2 (G) was quantified in the supernatants of drug-treated virus-infected cell cultures by titration in Vero cell cultures using the CPE reduction assay (TCID<sub>50</sub>/ml). The inhibition of viral replication was expressed in percentages and calculated with respect to the virus production in infected untreated macrophages. The inhibition of cytopathicity by the drugs was evaluated from microscopic inspection and was in agreement with the dose-dependent reduction of virus production. The data shown are from one representative experiment that was independently repeated three times.</p>
2013-07-25 02:07:19
Drugs and devices
Drug research and development
drug discovery
Infectious diseases
Viral diseases
Herpes simplex
hiv
pmeo-dapym
hsv-2
cultures
cpe
assay
pfu