10.1371/journal.ppat.1003530.g001
Muhamuda Kader
Muhamuda
Kader
Amanda P. Smith
Amanda
P. Smith
Cristiana Guiducci
Cristiana
Guiducci
Elizabeth R. Wonderlich
Elizabeth
R. Wonderlich
Daniel Normolle
Daniel
Normolle
Simon C. Watkins
Simon C.
Watkins
Franck J. Barrat
Franck
J. Barrat
Simon M. Barratt-Boyes
Simon
M. Barratt-Boyes
TLR7 and TLR9 antagonist selectively blocks proinflammatory cytokine production from uninfected rhesus macaque pDC.
Public Library of Science
2013
Infectious diseases
Viral diseases
hiv
tlr9
antagonist
selectively
blocks
proinflammatory
cytokine
uninfected
rhesus
macaque
2013-07-25 01:47:11
Figure
https://plos.figshare.com/articles/figure/_TLR7_and_TLR9_antagonist_selectively_blocks_proinflammatory_cytokine_production_from_uninfected_rhesus_macaque_pDC_/754822
<p>(A) Representative gating strategy to define pDC, mDC, monocytes (Mo) and macrophages (Mø) by flow cytometry in rhesus macaque PBMC and lymph node. The lymph node gate to define DC includes autofluorescent cells that encroach on the Lineage+ subset, as previously described <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003530#ppat.1003530-Brown1" target="_blank">[23]</a>. (B) PBMC and lymph node cell suspensions from SIV-naive animals were stimulated with iSIV or influenza virus with and without DV056 and intracellular expression of IFN-α and TNF-α in pDC was determined by flow cytometry. Positive labeling with cytokine-specific antibody as shown by the boxed areas was based on isotype control antibody. Numbers represent the percent positive cells. (C) PBMC, pDC-depleted PBMC and PBMC pretreated with DV056 were stimulated with iSIV, live SIV, influenza virus or CpG-C and supernatants were analyzed for IFN-α by ELISA. Unstimulated PBMC served as a negative control. (D) PBMC and lymph node cell suspensions were stimulated with iSIV or influenza virus and IFN-α and/or TNF-α in mDC, monocytes or macrophages were determined by flow cytometry. SSC = side scatter.</p>