10.1371/journal.pone.0069866
Simone Macrì
Simone
Macrì
Riccardo Sgarra
Riccardo
Sgarra
Gloria Ros
Gloria
Ros
Elisa Maurizio
Elisa
Maurizio
Salvina Zammitti
Salvina
Zammitti
Ornella Milani
Ornella
Milani
Marco Onorati
Marco
Onorati
Robert Vignali
Robert
Vignali
Guidalberto Manfioletti
Guidalberto
Manfioletti
Expression and Functional Characterization of <i>Xhmg-at-hook</i> Genes in <i>Xenopus laevis</i>
Public Library of Science
2013
Anatomy and physiology
Neurological system
Central nervous system
Biochemistry
proteins
DNA-binding proteins
Nucleic acids
developmental biology
Cell fate determination
embryology
genetics
gene expression
Gene function
Model organisms
Animal models
Xenopus laevis
characterization
genes
2013-07-25 01:44:51
Dataset
https://plos.figshare.com/articles/dataset/Expression_and_Functional_Characterization_of_Xhmg_at_hook_Genes_in_Xenopus_laevis_/754817
<div><p>High Mobility Group A proteins (HMGA1 and HMGA2) are architectural nuclear factors involved in development, cell differentiation, and cancer formation and progression. Here we report the cloning, developmental expression and functional analysis of a new multi-AT-hook factor in <i>Xenopus laevis</i> (XHMG-AT-hook) that exists in three different isoforms. <i>Xhmg-at-hook1</i> and <i>3</i> isoforms, but not isoform <i>2</i>, are expressed throughout the entire development of <i>Xenopus</i>, both in the maternal and zygotic phase. Localized transcripts are present in the animal pole in the early maternal phase; during the zygotic phase, mRNA can be detected in the developing central nervous system (CNS), including the eye, and in the neural crest. We show evidence that XHMG-AT-hook proteins differ from typical HMGA proteins in terms of their properties in DNA binding and in protein/protein interaction. Finally, we provide evidence that they are involved in early CNS development and in neural crest differentiation.</p></div>