%0 Figure %A Macrì, Simone %A Sgarra, Riccardo %A Ros, Gloria %A Maurizio, Elisa %A Zammitti, Salvina %A Milani, Ornella %A Onorati, Marco %A Vignali, Robert %A Manfioletti, Guidalberto %D 2013 %T XLHMGA2 and XHMG-AT-hook1 DNA-binding properties. %U https://plos.figshare.com/articles/figure/_XLHMGA2_and_XHMG_AT_hook1_DNA_binding_properties_/754813 %R 10.1371/journal.pone.0069866.g004 %2 https://ndownloader.figshare.com/files/1130636 %K Anatomy and physiology %K Neurological system %K Central nervous system %K Biochemistry %K proteins %K DNA-binding proteins %K Nucleic acids %K developmental biology %K Cell fate determination %K embryology %K genetics %K gene expression %K Gene function %K Model organisms %K Animal models %K Xenopus laevis %K xhmg-at-hook1 %K dna-binding %X

(A) Electrophoretic mobility shift assay performed with in vitro transcribed and translated (IVT) HA-tagged XLHMGA2βa (HA-XLA2ßa) and XHMG-AT-hook1 (HA–XATH1) proteins. Two different DNA probes were used: upper panel, E3 (0.1 pmoles); lower panel HCRII (0.1 pmoles); EMSAs were performed incubating 2, 4, and 6 µL of IVT proteins. (B) Western blot analysis of IVT proteins is shown (red ponceau stained membrane (left) and α-HA antibody recognition (right) to assess the production of the XLHMGA2βa and XHMG-AT-hook1 proteins.

%I PLOS ONE