HuR expression was not affected by Src, Raf1 and CaseinI inhibition in U937 monocytic cells.
Anna S. Sahlberg
Marja Ruuska
Kaisa Granfors
Markus A. Penttinen
10.1371/journal.pone.0070377.g004
https://plos.figshare.com/articles/figure/_HuR_expression_was_not_affected_by_Src_Raf1_and_CaseinI_inhibition_in_U937_monocytic_cells_/750329
<p>Full length HuR (36 kDa) and HuR cleavage products (CP1; 24 kDa and CP2; 8 kDa) in LPS-stimulated U937 Mock, B27g, H9F and E45M cells at 5 h time point. In A, p38 inhibitor BIRB796 (Birb) was incubated with the cells 30, 60 or 90 min prior to LPS stimulation. In B, Src, Casein I (Cas) and Raf1 kinase inhibitors were incubated with the cells 15 min prior to LPS stimulation. Expression of HuR was determined by SDS-PAGE and Western blotting. HSPA8 was used as a loading control. Results from densitometric analysis of five independent experiments are shown in diagram. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070377#s2" target="_blank">Materials and Methods</a> for details. * = P-value ≤0.06 for HuR, <sup>‡</sup> = P-value ≤0.06 for CP1 and <sup>†</sup> = P-value ≤0.06 for CP2 versus untreated sample at the respective cell line.</p>
2013-07-22 02:56:54
immunology
Immune cells
monocytes
Immune system
cytokines
Immune response
Major histocompatibility complex
microbiology
Bacterial pathogens
Salmonella
Host-pathogen interaction
Molecular cell biology
cytometry
flow cytometry
Signal transduction
Signaling cascades
MAPK signaling cascades
Protein kinase signaling cascade
Stress signaling cascade
Cellular stress responses
Infectious diseases
Bacterial diseases
affected
raf1
caseini
inhibition
u937
monocytic