HuR expression was not affected by Src, Raf1 and CaseinI inhibition in U937 monocytic cells. Anna S. Sahlberg Marja Ruuska Kaisa Granfors Markus A. Penttinen 10.1371/journal.pone.0070377.g004 https://plos.figshare.com/articles/figure/_HuR_expression_was_not_affected_by_Src_Raf1_and_CaseinI_inhibition_in_U937_monocytic_cells_/750329 <p>Full length HuR (36 kDa) and HuR cleavage products (CP1; 24 kDa and CP2; 8 kDa) in LPS-stimulated U937 Mock, B27g, H9F and E45M cells at 5 h time point. In A, p38 inhibitor BIRB796 (Birb) was incubated with the cells 30, 60 or 90 min prior to LPS stimulation. In B, Src, Casein I (Cas) and Raf1 kinase inhibitors were incubated with the cells 15 min prior to LPS stimulation. Expression of HuR was determined by SDS-PAGE and Western blotting. HSPA8 was used as a loading control. Results from densitometric analysis of five independent experiments are shown in diagram. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070377#s2" target="_blank">Materials and Methods</a> for details. * = P-value ≤0.06 for HuR, <sup>‡</sup> = P-value ≤0.06 for CP1 and <sup>†</sup> = P-value ≤0.06 for CP2 versus untreated sample at the respective cell line.</p> 2013-07-22 02:56:54 immunology Immune cells monocytes Immune system cytokines Immune response Major histocompatibility complex microbiology Bacterial pathogens Salmonella Host-pathogen interaction Molecular cell biology cytometry flow cytometry Signal transduction Signaling cascades MAPK signaling cascades Protein kinase signaling cascade Stress signaling cascade Cellular stress responses Infectious diseases Bacterial diseases affected raf1 caseini inhibition u937 monocytic