The inhibitory effect of IPA-3 on NF-κB nuclear translocation.
Leo Lap-Yan Wong
Ian Pak-Yan Lam
Tracy Yuk-Nar Wong
Wai-Lung Lai
Heong-Fai Liu
Lam-Lung Yeung
Yick-Pang Ching
10.1371/journal.pone.0068843.g005
https://plos.figshare.com/articles/figure/_The_inhibitory_effect_of_IPA_3_on_NF_954_B_nuclear_translocation_/748985
<p>(<b>A</b>) Effect of IPA-3 on subcellular localization of NF-κB was evaluated by immunofluorescence staining. After overnight serum starvation, H2M (left panel) and MIHA (right panel) cells were treated with either DMSO or IPA-3 (20 µM, 15 minutes) followed by an addition of TNF-α (20 ng/ml, 15 minutes). NF-κB was detected with a specific antibody (Green) and nucleus was stained with DAPI (Blue). (<b>B</b>) Western blotting analysis of P-PAK1 (T423) and total PAK1 were detected in the H2M cells stimulated by TNF-α (20 ng/ml) with or without IPA-3 pretreatment (20 µM, 15 minutes). TNF-α was included in the culture medium for 0, 0.5, 1, 2 or 4 hours. (<b>C</b>) Expression of quantitative real-time PCR was performed to analyze the mRNA level of MMP-9 (left panel) and COX-2 (right panel). Serum-starved H2M cells were treated with or without IPA-3 pretreatment (10 or 20 µM, 15 minutes) followed by TNF-α (10 or 20 ng/ml, 24 hours). Quantitative results of MMP-9 and COX-2 mRNA levels were normalized to β-actin. The values represented the mean ± SD of three independent experiments. *<i>P</i><0.001 (ANOVA), ***<i>P</i><0.05 (ANOVA) compared with the TNF-α control.</p>
2013-07-19 01:43:32
oncology
inhibitory
ipa-3