Co-immunoprecipitation of SOLO and SMC1 from ovarian extracts. Rihui Yan Bruce D. McKee 10.1371/journal.pgen.1003637.g003 https://plos.figshare.com/articles/figure/_Co_immunoprecipitation_of_SOLO_and_SMC1_from_ovarian_extracts_/748299 <p>Ovary lysates were prepared from <i>y w</i> and FH::SOLO (<i>{UASp-FH::SOLO}/CyO; {nos-GAL4::VP16}/TM2</i>) ovaries and used for Western blot and immunoprecipitation experiments. (A) Western blot of protein extracts from FH::SOLO and <i>y w</i> ovaries using anti-FLAG antibody. Absence of signal in the <i>y w</i> lane shows that the anti-FLAG antibody is specific. (B) Immunoprecipitation with anti-SMC1 antibody from protein extracts from FH::SOLO and <i>y w</i> ovaries, analyzed with anti-FLAG antibody. (C) Immunoprecipitation with anti-SMC1 antibody or rabbit serum (mimic control) from FH::SOLO ovary extracts, analyzed with anti-FLAG antibody. (D) Immunoprecipitation with anti-FLAG antibody or mouse serum (mimic control) from FH::SOLO ovary extracts, analyzed with anti-SMC1 antibody.</p> 2013-07-18 03:49:41 genetics Molecular cell biology solo smc1 ovarian