Manganese pretreatment offers no protection against the effects of Stx in Vero cells. Marsha A. Gaston Christine A. Pellino Alison A. Weiss 10.1371/journal.pone.0069823.g002 https://plos.figshare.com/articles/figure/_Manganese_pretreatment_offers_no_protection_against_the_effects_of_Stx_in_Vero_cells_/746651 <p>One hundred percent protein synthesis corresponds to cells incubated with no exogenous MnCl<sub>2</sub> and no toxin. All points were assayed in triplicate and error bars indicate standard deviation. (<b>A, B</b>) Percent protein synthesis of Vero cells incubated in the presence or absence of 250 µM MnCl<sub>2</sub> for 4 hours followed by an additional 4 hour incubation at 125 µM MnCl<sub>2</sub> with increasing concentrations of either (<b>A</b>) Stx1-S or (<b>B</b>) Stx2a.</p> 2013-07-16 03:20:51 microbiology Bacterial pathogens Escherichia coli Microbial pathogens Model organisms Animal models mouse toxicology Toxic agents Infectious diseases Bacterial diseases Hemolytic-uremic syndrome pretreatment offers stx vero