Impaired responses of cd44<sup>-/-</sup> bone marrow-derived macrophages. Kristin Schultz Christina Grieger (Lindner) Yong Li Pavel Urbánek Anne Ruschel Kerstin Minnich Dunja Bruder Marcus Gereke Antonio Sechi Peter Herrlich 10.1371/journal.pone.0207358.g007 https://plos.figshare.com/articles/figure/Impaired_responses_of_cd44_sup_-_-_sup_bone_marrow-derived_macrophages_/7457162 <p>BMDMs were isolated and differentiated from wild type control and knockout animals. <i>L</i>. <i>monocytogenes</i> were grown to an OD of ∼ 2.2. Bacteria were diluted 1:10.000 (low) or 1:5.000 (high) in infection media and introduced to macrophages as described in Materials and Methods. Cells were harvested 2 and 6 h after infection and analysed by RT-qPCR with <b>(A)</b> murine IFI16 oligos (primers 11 and 12, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0207358#pone.0207358.t001" target="_blank">Table 1</a>) or <b>(B)</b> IFN-β oligos (primers 13 and 14, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0207358#pone.0207358.t001" target="_blank">Table 1</a>) or <b>(C)</b> iNOS oligos (primers 15 and 16, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0207358#pone.0207358.t001" target="_blank">Table 1</a>). Experiments were done in triplicates. For statistics see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0207358#sec002" target="_blank">Materials and methods</a>.</p> 2018-12-12 18:54:00 CD 44 cleavage CD 44 molecule bone marrow-derived macrophages CD 44-ICD MEF CD 44-dependent transcriptional increase receptor tyrosine kinases IFI 16. IFI 16 IFN cd 44 gene disruption CD 44 cytoplasmic tail upregulates IFI 16 CD 44 RNA CD 44 domain DNA RPM-MC IFI 16 expression CD 44-negative tumor cell line