10.6084/m9.figshare.7430225.v1
Usmara Riaz Butt
Usmara Riaz
Butt
Rabia Naz
Rabia
Naz
Asia Nosheen
Asia
Nosheen
Humaira Yasmin
Humaira
Yasmin
Rumana Keyani
Rumana
Keyani
Ishtiaq Hussain
Ishtiaq
Hussain
Muhammad Nadeem Hassan
Muhammad Nadeem
Hassan
Changes in pathogenesis-related gene expression in response to bioformulations in the apoplast of maize leaves against <i>Fusarium oxysporum</i>
Taylor & Francis Group
2018
Wilting
methyl jasmonate
chitosan
plant extracts
defense enzymes
gene expression
2018-12-06 16:39:26
Journal contribution
https://tandf.figshare.com/articles/journal_contribution/Changes_in_pathogenesis-related_gene_expression_in_response_to_bioformulations_in_the_apoplast_of_maize_leaves_against_i_Fusarium_oxysporum_i_/7430225
<p>In present study, we initially tested four different plant species <i>Aristolochia indica</i> (leaf)<i>, Cuscuta pedicellata</i> (stem)<i>, Melilotus indicus</i> (leaf) and <i>Tribulus terresteris</i> (leaf and fruit) for their antifungal potential against <i>F. oxysporum.</i> Among the studied plant species, <i>T. terresteris</i> (fruit) and <i>C. pedicellate</i> showed the maximum antifungal potential by 92% and 89%, respectively. We then investigated the potential of CHI, MeJA, and plant extracts (<i>C. pedicellate</i> and <i>T. terristris</i>) alone as well as in combinations in pot experiment. Treatments with the extracts of <i>C. pedicellata</i> and <i>T. terresteris</i> in combination with MeJA resulted in decrease in disease severity by 93% and 89%, respectively, and enhanced the plant growth. We observed significant elevated levels of expression of pathogenesis-related (PR-1) proteins (β-1-3-glucanase, chitinase, phenyl-alanine ammonia lyase (PAL), lipoxygenase, lipid transfer protein and PR protein) and defense enzymes (β-1-3-glucanase, chitinase, peroxidase, polyphenoloxidase and PAL) in the CHI and MeJA in combination with plant extracts-treated plants for defense signaling pathways in the apoplast of maize leaves compared to the inoculated control. We found CHI in combination with <i>C. pedicellata</i> most effectively induced the plant defense mechanisms against <i>F. oxysporum</i> through increased induction in the level of expression of PR-proteins.</p>