%0 Figure %A Welles, Hugh C. %A F. Jennewein, Madeleine %A D. Mason, Rosemarie %A Narpala, Sandeep %A Wang, Lingshu %A Cheng, Cheng %A Zhang, Yi %A Todd, John-Paul %A D. Lifson, Jeffrey %A B. Balazs, Alejandro %A Alter, Galit %A B. McDermott, Adrian %A Mascola, John R. %A Roederer, Mario %D 2018 %T AAV-expressed mAb exhibit a distinct glycosylation profile from in vitro purified or naturally secreted mAb. %U https://plos.figshare.com/articles/figure/AAV-expressed_mAb_exhibit_a_distinct_glycosylation_profile_from_i_in_vitro_i_purified_or_naturally_secreted_mAb_/7427294 %R 10.1371/journal.ppat.1007395.g005 %2 https://ndownloader.figshare.com/files/13752515 %K AAV-directed muscle cell expression %K anti-transgene antibody response %K target antibody abundance %K AAV-based approaches %K Vectored delivery %K autologous anti-SIV neutralizing antibodies %K immunization strategy %K animal model %K Vectored transgene expression %K plasma expression %K mucosal accumulation %K transgene mAb %K dose SIVsmE 660 swarm challenge %K dose intrarectal swarm SIVsmE 660 challenge Gene %K injection %K glycosylation profiles %K anti-SIV envelope %K anti-vector antibody responses %K AAV 8 %K nonhuman primate model %K Adeno-Associated Virus 8 %K HIV %K gene therapy-based %K immunity %K 2 x 10 12 AAV 8 genome copies %K mucosal surfaces %K rhesus macaques %X

Glycans were eluted from serum anti-SIV env reactive mAbs from passively immunized or SIV-infected animals and analyzed via capillary electrophoresis. (A-D) Analysis of total grouped glycan species are displayed as a percent of total glycans (E) Individual glycan species of are quantified as a percent of all glycans and compared by derivation. (F-I) Analysis of total grouped glycan species of AAV expressed NAbs are compared across time. (J) Individual glycan species of are quantified as a percent of all glycans and compared across time for AAV derived antibodies. (A-D, F-I) Differences between groups reaching p ≤ 0.001 are denoted by black lines. (E, J) Differences between groups of individual glycans reaching p ≤ 0.001 are described in text.

%I PLOS Pathogens