Hoffmann-Vold, Anna-Maria Weigt, Stephen Samuel Palchevskiy, Vyacheslav Volkmann, Elizabeth Saggar, Rajan Li, Ning Midtvedt, Øyvind Lund, May Brit Garen, Torhild C. Fishbein, Michael Ardehali, Abbas Ross, David J. Ueland, Thor Aukrust, Pål Lynch, Joseph P. M. Elashoff, Robert Molberg, Øyvind A. Belperio, John Immunostaining of CX<sub>3</sub>CL1 and CX<sub>3</sub>CR1 in SSc-ILD. <p>CX<sub>3</sub>CL1 protein is produced from the epithelium and infiltrating interstitial leukocytes in SSc-ILD, while its receptor CX<sub>3</sub>CR1 is localized to infiltrating mononuclear cells. Representative histopathological staining of (n = 5) SSc-ILD for CX<sub>3</sub>CL1 from (A) Type 2 pneumocytes, (B) Airway epithelium, (C) Epithelium involved in bronchiolization, and (D) Infiltrating mononuclear cells. Representative histopathological staining of (n = 5) SSc-ILD for CX<sub>3</sub>CR1 from (E) Infiltrating interstitial mononuclear cells, (F) Morphologically from plasma cells via their eccentric cartwheel nuclei, (G) Representative staining of plasma cell marker CD138 confirming that the cells with eccentric cartwheel nuclei are plasma cells, (H) Representative immunofluorescence staining of plasma cell (red) in the interstitium, CX<sub>3</sub>CR1 positive infiltrating mononuclear cells, and co-localization of CD138<sup>+</sup> plasma cell expressing CX<sub>3</sub>CR1 and infiltrating the interstitium.</p> Results CX 3 CL 1 concentrations;ILD progression;CX 3 CL 1;multivariable regression analysis;Conclusion CX 3 CL 1;lung tissue;reactive type II pneumocytes;sclerosis Background Dysregulation;Oslo University Hospital SSc cohort;receptor CX 3 CR 1;ELISA;DLCO;lung disease;UCLA;airway epithelial cells;antibody secreting plasma cells;CX 3 CR 1;onset PH endpoints 2018-11-20
    https://plos.figshare.com/articles/figure/Immunostaining_of_CX_sub_3_sub_CL1_and_CX_sub_3_sub_CR1_in_SSc-ILD_/7365590
10.1371/journal.pone.0206545.g002