10.1371/journal.pone.0206251.g001 Pratap Karki Pratap Karki Angelo Meliton Angelo Meliton Alok Shah Alok Shah Yufeng Tian Yufeng Tian Tomomi Ohmura Tomomi Ohmura Nicolene Sarich Nicolene Sarich Anna A. Birukova Anna A. Birukova Konstantin G. Birukov Konstantin G. Birukov PM causes endothelial barrier disruption. Public Library of Science 2018 PGPC phospholipid POVPC mass spectrometry analysis cell barrier disruption Tr-OxPL cell junctions lung barrier dysfunction barrier dysfunction matter Particulate matter intracellular type 2 platelet-activating factor acetylhydrolase PAFAH PM-induced generation mechanism 2018-11-12 18:40:51 Figure https://plos.figshare.com/articles/figure/PM_causes_endothelial_barrier_disruption_/7328351 <p>(<b>A</b>) Human pulmonary lung EC were exposed to indicated concentrations of PM and TER was measured across the cell monolayers over time. (<b>B</b>) Cells grown on immobilized biotinylated gelatin were exposed to PM for 4 hr and FITC-avidin (25 μg/mL) was added for 3 min. After washing unbound FITC-avidin with PBS, FITC fluorescence was determined in Victor X5 plate reader. Normalized readings are expressed as mean ± S.D.; n = 6, *p<0.05. (<b>C</b>) Visualization of FITC fluorescence in control or PM-treated cells (20 μg/cm<sup>2</sup>, 4 h).</p>