10.1371/journal.pone.0206251.g001
Pratap Karki
Pratap
Karki
Angelo Meliton
Angelo
Meliton
Alok Shah
Alok
Shah
Yufeng Tian
Yufeng
Tian
Tomomi Ohmura
Tomomi
Ohmura
Nicolene Sarich
Nicolene
Sarich
Anna A. Birukova
Anna
A. Birukova
Konstantin G. Birukov
Konstantin
G. Birukov
PM causes endothelial barrier disruption.
Public Library of Science
2018
PGPC
phospholipid
POVPC
mass spectrometry analysis
cell barrier disruption
Tr-OxPL
cell junctions
lung barrier dysfunction
barrier dysfunction
matter Particulate matter
intracellular type 2 platelet-activating factor acetylhydrolase
PAFAH
PM-induced generation
mechanism
2018-11-12 18:40:51
Figure
https://plos.figshare.com/articles/figure/PM_causes_endothelial_barrier_disruption_/7328351
<p>(<b>A</b>) Human pulmonary lung EC were exposed to indicated concentrations of PM and TER was measured across the cell monolayers over time. (<b>B</b>) Cells grown on immobilized biotinylated gelatin were exposed to PM for 4 hr and FITC-avidin (25 μg/mL) was added for 3 min. After washing unbound FITC-avidin with PBS, FITC fluorescence was determined in Victor X5 plate reader. Normalized readings are expressed as mean ± S.D.; n = 6, *p<0.05. (<b>C</b>) Visualization of FITC fluorescence in control or PM-treated cells (20 μg/cm<sup>2</sup>, 4 h).</p>