10.1021/acs.inorgchem.8b02240.s001
Mostakim SK
Mostakim
SK
Sooram Banesh
Sooram
Banesh
Vishal Trivedi
Vishal
Trivedi
Shyam Biswas
Shyam
Biswas
Selective and Sensitive Sensing of Hydrogen Peroxide
by a Boronic Acid Functionalized Metal–Organic Framework and
Its Application in Live-Cell Imaging
American Chemical Society
2018
HEPES buffer
H 2 O 2 solution
probe
detection limit 0.015 μ M
framework
acid
ROS
imaging
reactive oxygen species
MOF
intracellular H 2 O 2
BDC-B
sensitivity
UiO -66 structure
H 2 O 2
Zr-UiO -66-B 2 compound
MDAMB -231 cells
2018-11-08 20:13:27
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Selective_and_Sensitive_Sensing_of_Hydrogen_Peroxide_by_a_Boronic_Acid_Functionalized_Metal_Organic_Framework_and_Its_Application_in_Live-Cell_Imaging/7318571
A new
boronic acid functionalized Zr(IV) metal–organic framework
having the capability of sensing H<sub>2</sub>O<sub>2</sub> in live
cells is reported. The Zr-MOF bears a UiO-66 structure and contains
2-boronobenzene-1,4-dicarboxylic acid (BDC-B(OH)<sub>2</sub>) as a
framework linker. The activated Zr-UiO-66-B(OH)<sub>2</sub> compound
(called <b>1′</b>) is highly selective for the fluorogenic
detection of H<sub>2</sub>O<sub>2</sub> in HEPES buffer at pH 7.4,
even in the presence of interfering ROS (ROS = reactive oxygen species)
and other biologically relevant analytes. The fluorescent probe was
found to display extraordinary sensitivity for H<sub>2</sub>O<sub>2</sub> (detection limit 0.015 μM) in HEPES buffer, which represents
a lower value in comparison to those of the MOF probes documented
so far for sensing H<sub>2</sub>O<sub>2</sub> using other analytical
methods. Taking advantage of its high selectivity and sensitivity
for H<sub>2</sub>O<sub>2</sub> in HEPES buffer, the probe was successfully
employed for the imaging of intracellular H<sub>2</sub>O<sub>2</sub>. Imaging studies with MDAMB-231 cells revealed the emergence of
bright blue fluorescence after loading with probe <b>1′</b> and subsequent treatment with H<sub>2</sub>O<sub>2</sub> solution.