10.6084/m9.figshare.7313642.v1
Interacteam UNSW
Interacteam
UNSW
ALL RAW DATA FOR : Pathological mutations differentially affect the self-assembly and polymerisation of the the immune system signaling adaptor molecule MyD88
figshare
2018
myd88-mediated
Cell Signalling
Polymerisation
Biophysics
Molecular Biology
2018-11-08 05:39:16
Dataset
https://figshare.com/articles/dataset/ALL_RAW_DATA_FOR_Pathological_mutations_differentially_affect_the_self-assembly_and_polymerisation_of_the_the_immune_system_signaling_adaptor_molecule_MyD88/7313642
The
fluorescence time-traces obtained for the full-length MYD88, mutants of MYD88
and the separate domains of MyD88 from
single molecule fluorescence spectroscopy. For TIR domain alone the
fluctuations of intensity around the average value are limited, for the death
domain, small bursts of intensities can be detected. These fluorescence peaks
correspond to entries of single protein complexes, increasing the local number
of proteins for a brief period of time. The amplitude and duration of the
deviations from average are linked to the number of proteins co-diffusing in a
single complex and the physical size of the diffusion complex. For full-length
MyD88, we observe extremely bright and long-diffusing bursts of fluorescence. The
simplest analysis to quantify the presence of protein complexes is to calculate
the average brightness of the diffusing species, calculated from the measured
intensity values (I) as B=〖(Standard
deviation (I))〗^2/(average
(I)). FCS data in solution. Values are from repeated dilution experiments with
the various protein concentrations and corresponding brightness values obtained
plotted. NS >0.9999, ***P=0.0001, ****P<0.0001