Toraskar, Jimita Magnussen, Synnøve N Chawla, Konika Svineng, Gunbjørg Steigedal, Tonje S Dataset for: Nephronectin mediates p38 MAPK-induced cell viability via its integrin binding enhancer motif Nephronectin (NPNT) is an extracellular matrix (ECM) protein involved in kidney development. We recently reported intracellular NPNT as a potential prognostic marker in breast cancer and that NPNT promotes metastasis in an integrin-dependent manner. Here we used Reverse Phase Protein Array (RPPA) to analyze NPNT-triggered intracellular signaling in the 66cl4 mouse breast cancer cell line. The results showed that the integrin binding enhancer motif is important for the cellular effects upon NPNT interaction with its receptors, including phosphorylation of p38 mitogen activated protein kinase (MAPK). Furthermore, analysis using prediction tools suggests involvement of NPNT in promoting cell viability. In conclusion, our results indicate that NPNT, via its integrin binding motifs, promote cell viability through phosphorylation of p38 MAPK. Breast Cancer;Extracellular matrix;Nephronectin;Integrin;p38 MAPK;Cell viability;Stem Cells;Synthetic Biology;Molecular Biology;Developmental Biology;Structural Biology;Proteomics and Intermolecular Interactions (excl. Medical Proteomics);Evolutionary Biology;Signal Transduction;Cancer Cell Biology;Systems Biology;Bioinformatics;Biostatistics;Heat and Mass Transfer Operations 2018-11-15
    https://wiley.figshare.com/articles/dataset/Dataset_for_Nephronectin_mediates_p38_MAPK-induced_cell_viability_via_its_integrin_binding_enhancer_motif/7270889
10.6084/m9.figshare.7270889.v1