10.3389/fmicb.2018.02287.s004
Kelei Zhao
Kelei
Zhao
Lianming Du
Lianming
Du
Jiafu Lin
Jiafu
Lin
Yang Yuan
Yang
Yuan
Xiwei Wang
Xiwei
Wang
Bisong Yue
Bisong
Yue
Xinrong Wang
Xinrong
Wang
Yidong Guo
Yidong
Guo
Yiwen Chu
Yiwen
Chu
Yingshun Zhou
Yingshun
Zhou
Table_3_Pseudomonas aeruginosa Quorum-Sensing and Type VI Secretion System Can Direct Interspecific Coexistence During Evolution.XLSX
Frontiers
2018
Pseudomonas aeruginosa
quorum-sensing
T6SS
evolution
coexistence
microcommunity
2018-10-11 14:47:43
Dataset
https://frontiersin.figshare.com/articles/dataset/Table_3_Pseudomonas_aeruginosa_Quorum-Sensing_and_Type_VI_Secretion_System_Can_Direct_Interspecific_Coexistence_During_Evolution_XLSX/7195676
<p>It is reported that a wide range of bacterial infections are polymicrobial, and the members in a local microcommunity can influence the growth of neighbors through physical and chemical interactions. Pseudomonas aeruginosa is an important opportunistic pathogen that normally causes a variety of acute and chronic infections, and clinical evidences suggest that P. aeruginosa can be frequently coisolated with other pathogens from the patients with chronic infections. However, the interspecific interaction and the coexisting mechanism of P. aeruginosa with coinfecting bacterial species during evolution still remain largely unclear. In this study, the relationships of P. aeruginosa with other Gram-positive (Staphylococcus aureus) and Gram-negative (Klebsiella pneumoniae) are investigated by using a series of on-plate proximity assay, in vitro coevolution assay, and RNA-sequencing. We find that although the development of a quorum-sensing system contributes P. aeruginosa a significant growth advantage to compete with S. aureus and K. pneumoniae, the quorum-sensing regulation of P. aeruginosa will be decreased during evolution and thus provides a basis for the formation of interspecific coexistence. The results of comparative transcriptomic analyses suggest that the persistent survival of S. aureus in the microcommunity has no significant effect on the intracellular transcriptional pattern of P. aeruginosa, while a more detailed competition happens between P. aeruginosa and K. pneumoniae. Specifically, the population of P. aeruginosa with decreased quorum-sensing regulation can still restrict the proportion increase of K. pneumoniae by enhancing the type VI secretion system-elicited cell aggressivity during further coevolution. These findings provide a general explanation for the formation of a dynamic stable microcommunity consisting of more than two bacterial species, and may contribute to the development of population biology and clinical therapy.</p>