10.1371/journal.pone.0065969.g001
Joseph Zacharia
Joseph
Zacharia
Joseph R. H. Mauban
Joseph
R. H. Mauban
Hema Raina
Hema
Raina
Steven A. Fisher
Steven
A. Fisher
Withrow G. Wier
Withrow
G. Wier
Receptors for sympathetic neurotransmitters activate at least 80% of contractile tone in mouse femoral arteries <i>in vivo</i>.
Public Library of Science
2013
Anatomy and physiology
Musculoskeletal system
muscle
Muscle functions
cardiovascular system
neuroscience
neurophysiology
neuromuscular junction
cardiovascular
Cardiovascular imaging
Cardiovascular pharmacology
Vascular biology
Drugs and devices
sympathetic
neurotransmitters
activate
contractile
femoral
arteries
2013-06-12 02:06:57
Figure
https://plos.figshare.com/articles/figure/_Receptors_for_sympathetic_neurotransmitters_activate_at_least_80_of_contractile_tone_in_mouse_femoral_arteries_in_vivo_/717617
<p>(A) Upper panel: 5 (a–e) successive transverse line-scan images of a femoral artery <i>in vivo.</i> Artery diameter was obtained as the distance between the two peaks in fluorescence intensity, representing the fluorescence within the walls of the artery. Artery diameter was measured only during the final 10 seconds of a 10 min duration control period or exposure to an antagonist. There is thus a 10 minute gap between each image (a–e) and between each corresponding diameter trace in the lower panel. Lower panel: Artery diameter under each condition (a–e). (a) Control, represents artery in basal state of anesthetized animal. (b–e) Arterial diameter upon on cumulative exposure to RS79948 (0.1 µM) (b), Prazosin (0.1 µM) (c), Suramin (0.5 mM)+BIBP3226 (1 µM) and (e). 0[Ca<sup>2+</sup>]. (B) Average data from 6 arteries. Fractional diameter is the measured diameter divided by the passive diameter for each artery, measured in the presence of 0[Ca<sup>2+</sup>] solution. In the combined presence of α<sub>1</sub>-, α<sub>2</sub>- adrenoceptor, NPY<sub>1</sub> and P<sub>2X</sub> receptor blockers, active vascular tone was reduced to less than 20% of the control level. As shown later, α<sub>1</sub>-adrenoceptors are not fully blocked at the concentration of prazosin used (0.1 µM), and thus the tone activated by noradrenaline is greater than shown here. Significance of difference from previous drug treatment, <sup>*</sup><i>P</i><0.05, <sup>**</sup><i>P</i><0.01, <sup>***</sup><i>P</i><0.001 (ANOVA followed by Newman-Keuls multiple comparison test).</p>