Analysis of the binding property of selected recombinant peptides with human sera in an ELISA.
Stefan Chabierski
Gustavo R. Makert
Alexandra Kerzhner
Luisa Barzon
Petra Fiebig
Uwe G. Liebert
Anna Papa
Justin M. Richner
Matthias Niedrig
Michael S. Diamond
Giorgio Palù
Sebastian Ulbert
10.1371/journal.pone.0066507.g003
https://plos.figshare.com/articles/figure/_Analysis_of_the_binding_property_of_selected_recombinant_peptides_with_human_sera_in_an_ELISA_/717611
<p>30-mer peptides spanning the WNV-proteins capsid, prM/M and E, fused to GST, were incubated with human sera from outbreaks in Italy (I1-8), Greece (G1–G15) and USA (US1-2). Those peptides displaying signals with an OD >0,5 are shown (the total number of peptides is shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066507#pone.0066507.s001" target="_blank">Figure S1</a>).Values represent the absorption over cut-off (mean of four negative sera plus two standard deviations) and are derived from at least two independent experiments (performed in duplicate). Error bars represent the standard deviation. The background (binding of the serum to GST) was subtracted.</p>
2013-06-12 02:06:51
immunology
Immunologic techniques
immunoassays
Immune response
microbiology
Virology
Emerging viral diseases
Viral disease diagnosis
Clinical immunology
immunity
Humoral immunity
Infectious diseases
Viral diseases
West Nile fever
binding
recombinant
peptides
sera