%0 Figure %A Yang, Chih-Ya %A Chen, Jiun-Bo %A Tsai, Ting-Fen %A Tsai, Yi-Chen %A Tsai, Ching-Yen %A Liang, Pi-Hui %A Hsu, Tsui-Ling %A Wu, Chung-Yi %A G. Netea, Mihai %A Wong, Chi-Huey %A Hsieh, Shie-Liang %D 2013 %T CLEC4F is involved in the presentation of α-GalCer to NKT cells. %U https://plos.figshare.com/articles/figure/_CLEC4F_is_involved_in_the_presentation_of_945_GalCer_to_NKT_cells_/713011 %R 10.1371/journal.pone.0065070.g006 %2 https://ndownloader.figshare.com/files/1076092 %K Biochemistry %K proteins %K Immune system proteins %K developmental biology %K embryology %K genetics %K gene expression %K Gene function %K immunology %K Immune cells %K Molecular cell biology %K nkt %X

(A) The schematic structure of α-GalCer. (B) The binding curves were obtained from the function of Fc.CLEC4F concentration and fluorescence intensity determined from array images. (C) Competition experiment between solution and surface for Fc.CLEC4F binding to α-GalCer and three derivatives. At different concentration of the competitors, binding curves were obtained from the bound Fc.CLEC4F concentration and fluorescence. (D) Secretion of IFN-γ and IL-4 of NKT cells after incubation of α-GalCer presented by Kupffer cells isolated from wild-type and Clec4f−/− mice. Kupffer cells (1×105 cells) isolated form wild-type and Clec4f−/− littermates were treated with serial concentration of α-GalCer (10, 30, 100, 300 and 1000 ng/ml) then co-cultured with NKT cells (1×105 cells). The supernatant were collected at 72 h post-stimulation and detected the IL-4 and IFN-γ production by ELISA. (E) Effect of CD1d-blocking antibody in the α-GalCer presentation by wild-type and Clec4f−/− Kupffer cells. Kupffer cells were pretreated with CD1d blocking antibody. Three hours later, α-GalCer (300 ng/ml) was added and co-cultured with NKT cells. The supernatant were collected at 72 h post-stimulation and detected the IL-4 and IFN-γ production by ELISA.

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