10.1371/journal.pone.0065647.g001 Paramita Mandal Paramita Mandal Bornali Bhattacharjee Bornali Bhattacharjee Damayanti Das Ghosh Damayanti Das Ghosh Nidhu Ranjan Mondal Nidhu Ranjan Mondal Rahul Roy Chowdhury Rahul Roy Chowdhury Sudipta Roy Sudipta Roy Sharmila Sengupta Sharmila Sengupta Relative quantification of L2 mRNA expression. Public Library of Science 2013 microbiology Emerging infectious diseases Host-pathogen interaction pathogenesis Virology Population biology epidemiology Infectious disease epidemiology Infectious diseases Sexually transmitted diseases Human papillomavirus infection Viral diseases Obstetrics and gynecology Genitourinary infections oncology Cancers and neoplasms Gynecological tumors Cervical cancer urology quantification l2 mrna 2013-06-06 00:44:00 Figure https://plos.figshare.com/articles/figure/_Relative_quantification_of_L2_mRNA_expression_/712640 <p>(<b>A</b>) Amplification plot based on quantitative real time PCR of HPV16 L2 expression. L2 is transcribed in E2 intact/episomal (episomal or concomitant) but in E2 disrupted/integrated cases. (<b>B</b>) Dissociation curve depicting the first-derivative melting curve for the reaction characterizing the expression of L2 (Tm of 80.5°C). (<b>C</b>) Amplification plot based on quantitative real time PCR of ACTB expression. ACTB is expressed by both episomal and integrated HPV16 positive cases. (<b>D</b>) Dissociation curve depicting the first-derivative melting curve for the reaction characterizing the expression of ACTB (Tm of 81.0°C).</p>