10.6084/m9.figshare.7105130.v1 Jakob Weiszmann Jakob Weiszmann Lisa Fürtauer Lisa Fürtauer Wolfram Weckwerth Wolfram Weckwerth Thomas Nägele Thomas Nägele Dataset for: Vacuolar sucrose cleavage prevents limitation of cytosolic carbohydrate metabolism and stabilizes photosynthesis under abiotic stress Wiley 2018 Arabidopsis thaliana natural variation invertase kinetic modelling energy metabolism Stem Cells Biochemistry Molecular Biology Developmental Biology Structural Biology Synthetic Biology Proteomics and Intermolecular Interactions (excl. Medical Proteomics) Evolutionary Biology Signal Transduction Cancer Cell Biology Systems Biology Bioinformatics Heat and Mass Transfer Operations 2018-09-28 09:42:09 Dataset https://wiley.figshare.com/articles/dataset/Dataset_for_Vacuolar_sucrose_cleavage_prevents_limitation_of_cytosolic_carbohydrate_metabolism_and_stabilizes_photosynthesis_under_abiotic_stress/7105130 Stabilization of central carbohydrate metabolism plays a key role in plant stress response. Carbohydrates are substrate for numerous metabolic and stress-responsive reactions and have been shown to be involved in diverse signalling processes on a whole plant level. Regulation of enzymatic sucrose synthesis and degradation is well-known to be central to many stress-related processes as it significantly impacts stress tolerance. Leaf sucrose metabolism involves sucrose cleavage by invertases and ATP-consuming re-synthesis catalysed by hexokinase and sucrose phosphate synthase. These reactions establish a metabolic cycle. To study the physiological role of sucrose cycling, a kinetic model was developed to simulate dynamics of subcellular sugar concentrations in Arabidopsis thaliana under combined cold and high light stress. Model simulation revealed that subcellular reprogramming of invertase-driven sucrose cleavage varies substantially between natural accessions of Arabidopsis which differ in their cold tolerance levels. A stress-induced shift of sucrose cleavage from the cytosol into the vacuole could only be observed for the tolerant accession while the susceptible accession increased the cytosolic proportion of sucrose cleavage. Under stress, reduction of vacuolar invertase activity significantly affected maximum quantum yield of photosystem II and CO2 assimilation rates. While wild type plants circumvented a limitation of sucrose cleavage by increasing vacuolar invertase activity, mutant plants were not able to compensate their deficiency of vacuolar by cytosolic activity. Consequently, the capacity for cytosolic hexose generation was lower than for enzymatic hexose phosphorylation suggesting a role of vacuolar invertase activity in preventing a limitation in cytosolic hexose metabolism under stress.