TY - DATA T1 - MIEF1 Microprotein Regulates Mitochondrial Translation PY - 2018/09/14 AU - Annie Rathore AU - Qian Chu AU - Dan Tan AU - Thomas F. Martinez AU - Cynthia J. Donaldson AU - Jolene K. Diedrich AU - John R. Yates AU - Alan Saghatelian UR - https://acs.figshare.com/articles/journal_contribution/MIEF1_Microprotein_Regulates_Mitochondrial_Translation/7088726 DO - 10.1021/acs.biochem.8b00726.s001 L4 - https://ndownloader.figshare.com/files/13034750 KW - MIEF 1-MP KW - mitochondrial translation rate KW - MIEF 1-MP decreases KW - protein interaction partners KW - APEX 2. MIEF 1-MP localizes KW - MIEF 1-MP increases KW - mitochondrial elongation factor 1 microprotein KW - MPI KW - MIEF 1 Microprotein Regulates Mitochondrial Translation N2 - Recent technological advances led to the discovery of hundreds to thousands of peptides and small proteins (microproteins) encoded by small open reading frames (smORFs). Characterization of new microproteins demonstrates their role in fundamental biological processes and highlights the value in discovering and characterizing more microproteins. The elucidation of microprotein–protein interactions (MPIs) is useful for determining the biochemical and cellular roles of microproteins. In this study, we characterize the protein interaction partners of mitochondrial elongation factor 1 microprotein (MIEF1-MP) using a proximity labeling strategy that relies on APEX2. MIEF1-MP localizes to the mitochondrial matrix where it interacts with the mitochondrial ribosome (mitoribosome). Functional studies demonstrate that MIEF1-MP regulates mitochondrial translation via its binding to the mitoribosome. Loss of MIEF1-MP decreases the mitochondrial translation rate, while an elevated level of MIEF1-MP increases the translation rate. The identification of MIEF1-MP reveals a new gene involved in this process. ER -