Pha13 confers self-ubiquitination E3 ligase and ubiquitin binding ability.
Li Chang
Ho-Hsiung Chang
Jui-Che Chang
Hsiang-Chia Lu
Tan-Tung Wang
Duen-Wei Hsu
Yuh Tzean
An-Po Cheng
Yi-Shu Chiu
Hsin-Hung Yeh
10.1371/journal.ppat.1007288.g006
https://plos.figshare.com/articles/figure/Pha13_confers_self-ubiquitination_E3_ligase_and_ubiquitin_binding_ability_/7086605
<p>In vitro ubiquitination analysis was performed on recombinant proteins of Pha13, A20 mutant (Pha13A20m), AN1 mutant (Pha13AN1m), A20/AN1 double mutant (Pha13A20mAN1m) with or without FLAG-tagged ubiquitin (FLAG-Ub), ubiquitin-activating enzyme (E1), or ubiquitin-conjugating enzyme (E2). Ubiquitinated proteins were analyzed with immunoblotting using anti-FLAG antibodies. Coomassie staining was used as a loading control. The ubiquitinated Pha13 (Pha13-2Ub), E1 conjugated with one Ub (E1-Ub), E2 conjugated with one or two Ub (E2-Ub, E2-2Ub), and free Ub are indicated with arrow.</p>
2018-09-13 18:15:16
dicot Arabidopsis overexpressing orchid Pha 13
NPR 1-independent virus defense genes
Subcellular localization analysis
Pha 13
immunity Salicylic acid
Pha 13. Functional domain analysis
ubiquitin chain binding activity
yeast two-hybrid screening
1 domain
virus resistance
exogenous SA treatment
1 zinc finger domains
E 3 ligase
orchid NPR 1
AtSAP 5
20 domain
protein
master regulator gene
Pha 13. Pha 13
E 3 ligase activity