%0 Figure %A Chang, Li %A Chang, Ho-Hsiung %A Chang, Jui-Che %A Lu, Hsiang-Chia %A Wang, Tan-Tung %A Hsu, Duen-Wei %A Tzean, Yuh %A Cheng, An-Po %A Chiu, Yi-Shu %A Yeh, Hsin-Hung %D 2018 %T Identification of Pha13 involvement in PhaPR1 expression by CymMV-based VIGS system and sequence analysis. %U https://plos.figshare.com/articles/figure/Identification_of_Pha13_involvement_in_i_PhaPR1_i_expression_by_CymMV-based_VIGS_system_and_sequence_analysis_/7086590 %R 10.1371/journal.ppat.1007288.g001 %2 https://ndownloader.figshare.com/files/13029953 %K dicot Arabidopsis overexpressing orchid Pha 13 %K NPR 1-independent virus defense genes %K Subcellular localization analysis %K Pha 13 %K immunity Salicylic acid %K Pha 13. Functional domain analysis %K ubiquitin chain binding activity %K yeast two-hybrid screening %K 1 domain %K virus resistance %K exogenous SA treatment %K 1 zinc finger domains %K E 3 ligase %K orchid NPR 1 %K AtSAP 5 %K 20 domain %K protein %K master regulator gene %K Pha 13. Pha 13 %K E 3 ligase activity %X

(A) Expression level of Pha13, PhaPR1, and CymMV were analyzed by RT-PCR from leaves of P. aphrodite inoculated with buffer (Mock), or infiltrated with agrobacterium carrying pCymMV (Cy) or pCymMV-Pha13 (Pha13-VIGS). PhaUbiquitin 10 (PhaUBQ10) was used as a loading control, and the relative expression level of corresponding genes are indicated. (B) Domains of Pha13. Open rectangle indicates the entire protein. A20 (black rectangle) and AN1 (diagonally-striped rectangle), nuclear localization signal (open square with vertical lines), and 21-nucleotide position (short black line) used for designing hairpin RNA of Pha13 (hpPha13) are indicated. (C) Sequence alignment of A20/AN1 zinc finger domains of Pha13 with stress-associated proteins from Arabidopsis thaliana (AtSAP5, accession number: AT3G12630), and Oryza sativa (OsSAP3, accession number: LOC_Os01g56040.1; OsSAP5, accession number: LOC_Os02g32840.1) are shown. Conserved amino acid sequences are indicated with a black box. Conserved cysteine (C) and histidine (H) are indicated with a black triangle. (D) Primary sequence organization of A20 and AN1 domains. Xn: the number of amino acid residues between zinc ligands. (B to D) The mutation positions for domain functional analysis are indicated with red triangle.

%I PLOS Pathogens