Yin, Yanni Wu, Sisi Chui, Chaonan Ma, Tianling Jiang, Huixian Hahn, Matthias Ma, Zhonghua BcPro40 was identified as a scaffold protein for the MAPK cassette of the CWI pathway in <i>B</i>. <i>cinerea</i>. <p>(A) Structure sketches of BcPro40 used for the yeast two-hybrid analysis. (B) Yeast two-hybrid analysis of interaction of the MAP kinsese of CWI pathway or the PAS kinase BcRim15 with BcPro40 or BcPro40 derivatives. Yeast cells (10<sup>6</sup> cells ml<sup>–1</sup>) transferred with bait and prey constructs indicated in the figure were drop-plated on SD-Leu-Trp-His plates. A pair of plasmids pGBKT7-53 and pGADT7 was used as a positive control. A pair of plasmids pGBKT7-Lam and pGADT7 was used as a negative control. Empty squares indicate that interactions were not tested.</p> PAS;cell wall integrity;mitogen-activated protein kinase;MAPK kinase BcMkk 1;BcMkk 1;kinase BcRim 15;EH;controls OA production;MAPK kinase;Botrytis cinerea;virulence;Pro 40 homolog BcPro 40;oxalic acid biosynthesis;cell wall;CWI 2018-09-13
    https://plos.figshare.com/articles/figure/BcPro40_was_identified_as_a_scaffold_protein_for_the_MAPK_cassette_of_the_CWI_pathway_in_i_B_i_i_cinerea_i_/7086581
10.1371/journal.ppat.1007285.g006