E. Hopcraft, Sharon Pattenden, Samantha G. James, Lindsey I. Frye, Stephen P. Dittmer, Dirk Damania, Blossom Romidepsin, Panobinostat, and PTC-209 induce KSHV genome replication and release of infectious virions. <p>(A) qPCR analysis for cell-associated KSHV genome copies was performed on BCBL-1 cells treated with DMSO or 1 μM Romidepsin or Panobinostat for five days. Naïve Vero cells were infected with supernatants collected from BCBL-1 cells treated as in (A), and KSHV genome copies in the infected cells were determined by qPCR (B), and KSHV lytic transcript levels were assayed by RT-qPCR (C). Lytic transcript levels were normalized to GAPDH, and the fold difference between DMSO and compound treated cells was calculated. (D) qPCR analysis for cell-associated KSHV genome copies was performed on BCBL-1 cells treated with DMSO or 10 μM PTC-209 for seven days. Naïve Vero cells were infected with supernatants collected from BCBL-1 cells treated as in (D), and KSHV genome copies in the infected cells were determined by qPCR (E), and KSHV lytic transcript levels were assayed by RT-qPCR (F). Lytic transcript levels were normalized to GAPDH, and the fold difference between DMSO and PTC-209 treated cells was calculated.</p> Kaposi;JQ;histone deacetylase inhibitors;sarcoma-associated herpesvirus reactivation;lytic gene expression;B cell cancers;PTC;KSHV lytic gene transactivator RTA;Primary Effusion Lymphoma;Bmi;KSHV reactivation;host chromatin-modifying proteins 2018-09-13
    https://plos.figshare.com/articles/figure/Romidepsin_Panobinostat_and_PTC-209_induce_KSHV_genome_replication_and_release_of_infectious_virions_/7086548
10.1371/journal.ppat.1007267.g004