%0 Figure %A E. Hopcraft, Sharon %A Pattenden, Samantha G. %A James, Lindsey I. %A Frye, Stephen %A P. Dittmer, Dirk %A Damania, Blossom %D 2018 %T Romidepsin, Panobinostat, (+)-JQ1, and PTC-209 induce KSHV lytic gene expression. %U https://plos.figshare.com/articles/figure/Romidepsin_Panobinostat_-JQ1_and_PTC-209_induce_KSHV_lytic_gene_expression_/7086542 %R 10.1371/journal.ppat.1007267.g002 %2 https://ndownloader.figshare.com/files/13029842 %K Kaposi %K JQ %K histone deacetylase inhibitors %K sarcoma-associated herpesvirus reactivation %K lytic gene expression %K B cell cancers %K PTC %K KSHV lytic gene transactivator RTA %K Primary Effusion Lymphoma %K Bmi %K KSHV reactivation %K host chromatin-modifying proteins %X

(A) RT-qPCR analysis for KSHV lytic transcript levels was performed on PEL cells treated with DMSO, NaB+TPA, or 1 μM Romidepsin or Panobinostat for 48 hours. Lytic transcript levels were normalized to GAPDH, and the fold difference between DMSO and compound treated cells was calculated. (B) Immunoblot analysis for vIL-6, ORF45, or actin was performed on lysates from BCBL-1 cells treated as in (A). Approximate molecular mass (kDa) marker positions are indicated to the left of each blot. (C) RT-qPCR analysis for KSHV lytic transcript levels was performed as in (A) on PEL cells treated with DMSO, NaB+TPA, or 10 μM (+)-JQ1 or PTC-209 for 120 hours. (D) Immunoblot analysis was performed on lysates from BCBL-1 cells treated as in (C). Data are representative of at least two independent experiments performed in triplicate; mean and standard error are shown.

%I PLOS Pathogens