10.26188/5b8b4c18916ff
Lina Maria Martinez Lopez
Lina Maria
Martinez Lopez
The role of the intestinal microbiota in the pathogenesis of chronic enteropathies and their interplay with the immune system
The University of Melbourne
2018
Canine Chronic enteropathy
Immunoglobulin
TSLP
Assembly Gut Microbiota
Gut Microbiota
Veterinary Microbiology (excl. Virology)
Veterinary Medicine
2018-09-02 02:33:57
Dataset
https://melbourne.figshare.com/articles/dataset/The_role_of_the_intestinal_microbiota_in_the_pathogenesis_of_chronic_enteropathies_and_their_interplay_with_the_immune_system/7027997
<p></p><p>The intestinal microbiota and its associated genome is
collectively called the gastrointestinal (GI) microbiome; and is composed of
crucial components that help not only to determine host biology but also to
maintain host physiology. Dysregulation of the gastrointestinal microbiome has
been associated with a range of diseases in people such as inflammatory bowel
disease (IBD), diabetes and obesity. Previous studies have found dysbiosis and
a reduced bacterial diversity in dogs with chronic enteropathies (CE). However,
the precise nature of the intestinal microbiota dysfunction and whether the
microbiota has a causative role or is secondarily affected remain to be
elucidated.</p><p> </p><p>The first step in understanding the
relationship between the gut microbiota and disease is the characterisation of
the normal gut microbiota, how it is established and how stable it is during
different periods of life. In this work, we assessed the dynamics and stability
of faecal microbiota over time in healthy dogs of different age groups, and the
development of the microbiota from birth in puppies, and the association with
the maternal microbiome. Next, we characterised highly immunoglobulin A and G
coated bacteria in faecal samples from dogs with chronic enteropathies using
flow cytometry and 16S rRNA sequencing and assessed their correlation with
disease stage and resolution of the clinical signs. Finally, we characterised
the expression of thymic stromal lymphopoetin (TSLP), a cytokine that is
produced in response to bacterial contact, in the intestine of healthy dogs and
its correlation with disease activity in dogs with chronic enteropathies.</p><p> </p><p>The results reported here, help to understand the
assembly of the gut microbiota, its interaction with the immune system and
emphasise on the importance of longitudinal studies and personalised approach
in order to understand the pathogenesis and the role of the microbiota in
intestinal diseases in dogs.</p><p>
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