TY - DATA T1 - Schematic of the miR-155/miR-155* genomic locus and the location of the TALEN pair engineered to target the miR-155* region. PY - 2013/05/07 AU - Ruozhen Hu AU - Jared Wallace AU - Timothy J. Dahlem AU - David Jonah Grunwald AU - Ryan M. O'Connell UR - https://plos.figshare.com/articles/figure/_Schematic_of_the_miR_155_miR_155_genomic_locus_and_the_location_of_the_TALEN_pair_engineered_to_target_the_miR_155_region_/699102 DO - 10.1371/journal.pone.0063074.g001 L4 - https://ndownloader.figshare.com/files/1055305 KW - Biochemistry KW - Nucleic acids KW - rna KW - RNA interference KW - biotechnology KW - Genetic engineering KW - Computational biology KW - genomics KW - Functional genomics KW - Molecular genetics KW - Gene regulation KW - gene expression KW - genetics KW - Genetic mutation KW - mutagenesis KW - Gene function KW - Molecular cell biology KW - genomic KW - locus KW - talen KW - engineered N2 - (A) Schematic of the miR-155/miR-155* genomic locus. The three BIC exons are shown in yellow and the miR-155 hairpin is in red. (B) Schematic of the miR-155 hairpin structure. The miR-155 arms are shown in black, while the mature miR-155 and miR-155* sequences are in dark grey. Blue and green boxes represent the binding sites of the TALEN pair designed to target the miR-155* region, and hexagons represent the heterodimerized FokI enzyme positioned over the spacer sequence. (C) The two expression plasmids containing the TALEN pair along with the FokI nuclease domain, and the TALEN-RVD sequences corresponding to each targeted DNA sequence are shown. The details of pCS2TAL3-DDD and pCS2TAL3-RRR expression vectors are described in the Materials and Methods section. NN, HD, NG and NI represent the RVD regions of each repeat sequence that bind to nucleotide G, C, T and A, respectively. The left and right TALEN binding sequences are shown in red and purple, respectively, and the spacer region is in blue. ER -