10.1371/journal.pone.0063074.g001 Ruozhen Hu Ruozhen Hu Jared Wallace Jared Wallace Timothy J. Dahlem Timothy J. Dahlem David Jonah Grunwald David Jonah Grunwald Ryan M. O'Connell Ryan M. O'Connell Schematic of the miR-155/miR-155* genomic locus and the location of the TALEN pair engineered to target the miR-155* region. Public Library of Science 2013 Biochemistry Nucleic acids rna RNA interference biotechnology Genetic engineering Computational biology genomics Functional genomics Molecular genetics Gene regulation gene expression genetics Genetic mutation mutagenesis Gene function Molecular cell biology genomic locus talen engineered 2013-05-07 02:31:42 Figure https://plos.figshare.com/articles/figure/_Schematic_of_the_miR_155_miR_155_genomic_locus_and_the_location_of_the_TALEN_pair_engineered_to_target_the_miR_155_region_/699102 <p>(A) Schematic of the miR-155/miR-155* genomic locus. The three BIC exons are shown in yellow and the miR-155 hairpin is in red. (B) Schematic of the miR-155 hairpin structure. The miR-155 arms are shown in black, while the mature miR-155 and miR-155* sequences are in dark grey. Blue and green boxes represent the binding sites of the TALEN pair designed to target the miR-155* region, and hexagons represent the heterodimerized FokI enzyme positioned over the spacer sequence. (C) The two expression plasmids containing the TALEN pair along with the FokI nuclease domain, and the TALEN-RVD sequences corresponding to each targeted DNA sequence are shown. The details of pCS2TAL3-DDD and pCS2TAL3-RRR expression vectors are described in the Materials and Methods section. NN, HD, NG and NI represent the RVD regions of each repeat sequence that bind to nucleotide G, C, T and A, respectively. The left and right TALEN binding sequences are shown in red and purple, respectively, and the spacer region is in blue.</p>