CaSR expression in WKY- and SHR-vSMVCs. Natalia Di Pietro Maria Assunta Potenza Sara Di Silvestre Francesco Addabbo Nadia Di Pietrantonio Pamela Di Tomo Caterina Pipino Domitilla Mandatori Carola Palmerini Paola Failli Mario Bonomini Monica Montagnani Assunta Pandolfi 10.1371/journal.pone.0202354.g005 https://plos.figshare.com/articles/figure/CaSR_expression_in_WKY-_and_SHR-vSMVCs_/6952928 <p>(A) Representative morphometric aspect of confluent vSMC cultures from normotensive rats (WKY) and spontaneously hypertensive rats (SHR) (magnification x 10; scale bar: 300 μM). The cells were plated and morphologically examined three different times in the same experimental conditions. (B) Representative pattern of electrophoretic bands (170–100, 70–55 and 40–25 kDa) that results from immunoblotting analysis of CaSR expression in WKY- and SHR-vSMCs lysates and its negative (NC, HEK293 empty vector transfected control cell lysate) and positive (PC, HEK293 CaSR transiently transfected cell lysate) controls. (C) Representative images of α-SMA flow cytometry analysis in WKY-and SHR-vSMCs and secondary antibody alone (control).</p> 2018-08-09 17:58:23 L-NAME CaSR Spontaneously Hypertensive rats Calcimimetic R -568 vasodilatory effect SHR CTX muscle cells MVB eNOS phosphorylation levels R -568 concentration MBV calcimimetic R -568 WKY R -568 doses INDO