CaSR expression in WKY- and SHR-vSMVCs.
Natalia Di Pietro
Maria Assunta Potenza
Sara Di Silvestre
Francesco Addabbo
Nadia Di Pietrantonio
Pamela Di Tomo
Caterina Pipino
Domitilla Mandatori
Carola Palmerini
Paola Failli
Mario Bonomini
Monica Montagnani
Assunta Pandolfi
10.1371/journal.pone.0202354.g005
https://plos.figshare.com/articles/figure/CaSR_expression_in_WKY-_and_SHR-vSMVCs_/6952928
<p>(A) Representative morphometric aspect of confluent vSMC cultures from normotensive rats (WKY) and spontaneously hypertensive rats (SHR) (magnification x 10; scale bar: 300 μM). The cells were plated and morphologically examined three different times in the same experimental conditions. (B) Representative pattern of electrophoretic bands (170–100, 70–55 and 40–25 kDa) that results from immunoblotting analysis of CaSR expression in WKY- and SHR-vSMCs lysates and its negative (NC, HEK293 empty vector transfected control cell lysate) and positive (PC, HEK293 CaSR transiently transfected cell lysate) controls. (C) Representative images of α-SMA flow cytometry analysis in WKY-and SHR-vSMCs and secondary antibody alone (control).</p>
2018-08-09 17:58:23
L-NAME
CaSR
Spontaneously Hypertensive rats
Calcimimetic R -568 vasodilatory effect
SHR
CTX
muscle cells
MVB
eNOS phosphorylation levels
R -568 concentration
MBV
calcimimetic R -568
WKY
R -568 doses
INDO