10.1371/journal.pone.0202354.g003 Natalia Di Pietro Natalia Di Pietro Maria Assunta Potenza Maria Assunta Potenza Sara Di Silvestre Sara Di Silvestre Francesco Addabbo Francesco Addabbo Nadia Di Pietrantonio Nadia Di Pietrantonio Pamela Di Tomo Pamela Di Tomo Caterina Pipino Caterina Pipino Domitilla Mandatori Domitilla Mandatori Carola Palmerini Carola Palmerini Paola Failli Paola Failli Mario Bonomini Mario Bonomini Monica Montagnani Monica Montagnani Assunta Pandolfi Assunta Pandolfi Effect of endothelium removal and CaSR inhibition on vascular response to R-568. Public Library of Science 2018 L-NAME CaSR Spontaneously Hypertensive rats Calcimimetic R -568 vasodilatory effect SHR CTX muscle cells MVB eNOS phosphorylation levels R -568 concentration MBV calcimimetic R -568 WKY R -568 doses INDO 2018-08-09 17:58:23 Figure https://plos.figshare.com/articles/figure/Effect_of_endothelium_removal_and_CaSR_inhibition_on_vascular_response_to_R-568_/6952922 <p>(A) MVB isolated from 8-week old WKY (closed symbols) and SHR (open symbols) were stimulated with increasing concentrations of R-568 in the presence (circles) or in the absence (squares) of endothelium. Results shown are the mean ± SEM of 3 (WKY) and 3 (SHR) independent experiments. Data from each curve were normalized as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0202354#pone.0202354.g001" target="_blank">Fig 1</a>. (B) MVB isolated from 8-week old WKY (closed symbols) and SHR (open symbols) were stimulated with increasing concentrations of R-568 in the absence (circles) or after pretreatment with (diamonds) Calhex-231 (3 μM/20 min). Results are the mean ± SEM of 3 WKY and 3 SHR independent experiments. Data from each curve were normalized as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0202354#pone.0202354.g001" target="_blank">Fig 1</a>. *p < 0.05 <i>vs</i> respective CTRL curve.</p>