%0 Figure %A Di Pietro, Natalia %A Assunta Potenza, Maria %A Silvestre, Sara Di %A Addabbo, Francesco %A Di Pietrantonio, Nadia %A Di Tomo, Pamela %A Pipino, Caterina %A Mandatori, Domitilla %A Palmerini, Carola %A Failli, Paola %A Bonomini, Mario %A Montagnani, Monica %A Pandolfi, Assunta %D 2018 %T Vascular effects of R-568 in MVB from WKY and SHR rats. %U https://plos.figshare.com/articles/figure/Vascular_effects_of_R-568_in_MVB_from_WKY_and_SHR_rats_/6952916 %R 10.1371/journal.pone.0202354.g001 %2 https://ndownloader.figshare.com/files/12749750 %K L-NAME %K CaSR %K Spontaneously Hypertensive rats %K Calcimimetic R -568 vasodilatory effect %K SHR %K CTX %K muscle cells %K MVB %K eNOS phosphorylation levels %K R -568 concentration %K MBV %K calcimimetic R -568 %K WKY %K R -568 doses %K INDO %X

Mesenteric vascular beds (MVB) from 8-week old WKY and SHR rats were isolated and prepared as described in Methods. PP at approximately 120 mmHg was maintained by continuous infusion with NA (10 μM and 3 μM in WKY and SHR rats, respectively). (A) and (B) Representative tracings of vasodilator responses to submaximal ACh dose (1 μM) and to increasing concentrations of R-568 (10 nM—30 μM) in WKY (A) and SHR (B) are shown. Each symbol represents the start of a 4-min perfusion. (C) Dose-response curves for R-568-induced vascular effects were obtained from MVB of WKY and SHR. Results are the mean ± SEM of 7 (WKY) and 6 (SHR) independent experiments. Data from each curve were normalized by defining 100% as the initial steady state PP and 0% as the maximal reduction in PP obtained in WKY treated with 1 μM ACh. *p < 0.01 vs respective values.

%I PLOS ONE