10.1371/journal.pone.0202354.g001 Natalia Di Pietro Natalia Di Pietro Maria Assunta Potenza Maria Assunta Potenza Sara Di Silvestre Sara Di Silvestre Francesco Addabbo Francesco Addabbo Nadia Di Pietrantonio Nadia Di Pietrantonio Pamela Di Tomo Pamela Di Tomo Caterina Pipino Caterina Pipino Domitilla Mandatori Domitilla Mandatori Carola Palmerini Carola Palmerini Paola Failli Paola Failli Mario Bonomini Mario Bonomini Monica Montagnani Monica Montagnani Assunta Pandolfi Assunta Pandolfi Vascular effects of R-568 in MVB from WKY and SHR rats. Public Library of Science 2018 L-NAME CaSR Spontaneously Hypertensive rats Calcimimetic R -568 vasodilatory effect SHR CTX muscle cells MVB eNOS phosphorylation levels R -568 concentration MBV calcimimetic R -568 WKY R -568 doses INDO 2018-08-09 17:58:23 Figure https://plos.figshare.com/articles/figure/Vascular_effects_of_R-568_in_MVB_from_WKY_and_SHR_rats_/6952916 <p>Mesenteric vascular beds (MVB) from 8-week old WKY and SHR rats were isolated and prepared as described in Methods. PP at approximately 120 mmHg was maintained by continuous infusion with NA (10 μM and 3 μM in WKY and SHR rats, respectively). (A) and (B) Representative tracings of vasodilator responses to submaximal ACh dose (1 μM) and to increasing concentrations of R-568 (10 nM—30 μM) in WKY (A) and SHR (B) are shown. Each symbol represents the start of a 4-min perfusion. (C) Dose-response curves for R-568-induced vascular effects were obtained from MVB of WKY and SHR. Results are the mean ± SEM of 7 (WKY) and 6 (SHR) independent experiments. Data from each curve were normalized by defining 100% as the initial steady state PP and 0% as the maximal reduction in PP obtained in WKY treated with 1 μM ACh. *p < 0.01 <i>vs</i> respective values.</p>