10.1371/journal.pone.0062188.g002
Karen S. Hathcock
Karen
S. Hathcock
Steven Bowen
Steven
Bowen
Ferenc Livak
Ferenc
Livak
Richard J. Hodes
Richard
J. Hodes
ATM deficiency alters in-frame and out-of-frame junctional sequences of TCRβ CDR3 in pre- and post-selection T cells.
Public Library of Science
2013
Biochemistry
Nucleic acids
dna
DNA recombination
DNA repair
developmental biology
Cell differentiation
immunology
Genetics of the immune system
Immune system
immunity
Model organisms
Animal models
mouse
Molecular cell biology
deficiency
alters
in-frame
out-of-frame
junctional
sequences
cdr3
pre-
post-selection
2013-04-23 02:21:06
Figure
https://plos.figshare.com/articles/figure/_ATM_deficiency_alters_in_frame_and_out_of_frame_junctional_sequences_of_TCR_946_CDR3_in_pre_and_post_selection_T_cells_/688466
<p>(A) Diagram of TCRβ CDR3 junctions. Nucleotide deletions and additions were sequenced from in-frame and out-of-frame TCRβ CDR3 regions isolated from ATMWT (grey bars) or KO (white bars) cells that were either TCRα KO DP thymocytes (B and C) or naïve CD4<sup>+</sup> T cells (D and E). TCRαKO DP and naïve CD4<sup>+</sup> T cells were sorted from two mice of each ATM genotype. Each data point represents >55,000 sequences. P values were calculated using Student’s unpaired 2-tailed t-test. *p<0.05; **p<0.01; ***p<0.001.</p>