10.1371/journal.pone.0062188.g002 Karen S. Hathcock Karen S. Hathcock Steven Bowen Steven Bowen Ferenc Livak Ferenc Livak Richard J. Hodes Richard J. Hodes ATM deficiency alters in-frame and out-of-frame junctional sequences of TCRβ CDR3 in pre- and post-selection T cells. Public Library of Science 2013 Biochemistry Nucleic acids dna DNA recombination DNA repair developmental biology Cell differentiation immunology Genetics of the immune system Immune system immunity Model organisms Animal models mouse Molecular cell biology deficiency alters in-frame out-of-frame junctional sequences cdr3 pre- post-selection 2013-04-23 02:21:06 Figure https://plos.figshare.com/articles/figure/_ATM_deficiency_alters_in_frame_and_out_of_frame_junctional_sequences_of_TCR_946_CDR3_in_pre_and_post_selection_T_cells_/688466 <p>(A) Diagram of TCRβ CDR3 junctions. Nucleotide deletions and additions were sequenced from in-frame and out-of-frame TCRβ CDR3 regions isolated from ATMWT (grey bars) or KO (white bars) cells that were either TCRα KO DP thymocytes (B and C) or naïve CD4<sup>+</sup> T cells (D and E). TCRαKO DP and naïve CD4<sup>+</sup> T cells were sorted from two mice of each ATM genotype. Each data point represents >55,000 sequences. P values were calculated using Student’s unpaired 2-tailed t-test. *p<0.05; **p<0.01; ***p<0.001.</p>