10.1371/journal.pone.0061353.g004
Shalom Madar
Shalom
Madar
Einav Harel
Einav
Harel
Ido Goldstein
Ido
Goldstein
Yan Stein
Yan
Stein
Ira Kogan-Sakin
Ira
Kogan-Sakin
Iris Kamer
Iris
Kamer
Hilla Solomon
Hilla
Solomon
Elya Dekel
Elya
Dekel
Perry Tal
Perry
Tal
Naomi Goldfinger
Naomi
Goldfinger
Gilgi Friedlander
Gilgi
Friedlander
Varda Rotter
Varda
Rotter
Mutant p53 counteracts IFNβ by SOCS1-mediated attenuation of STAT1 phosphorylation.
Public Library of Science
2013
genetics
gene expression
DNA transcription
RNA stability
Cancer genetics
Gene networks
Genetic mutation
Genetics of disease
immunology
Immunologic subspecialties
Tumor immunology
Molecular cell biology
Signal transduction
Signaling in cellular processes
Antiapoptotic signaling
Signaling in selected disciplines
Oncogenic signaling
Clinical immunology
oncology
Cancers and neoplasms
Lung and intrathoracic tumors
Adenocarcinoma of the lung
p53
counteracts
socs1-mediated
attenuation
stat1
2013-04-22 02:10:40
Figure
https://plos.figshare.com/articles/figure/_Mutant_p53_counteracts_IFN_946_by_SOCS1_mediated_attenuation_of_STAT1_phosphorylation_/687840
<p>(A) Cells were treated with IFNβ for 16 h, fixed and sorted by an “Image stream” FACS. The upper panel depicts representative images from each condition and the graph represents the mean pixel intensity of STAT1 positive cells for the entire population. (B) Same as in A, here the graph shows the similarity between p-STAT and DAPI staining, thereby quantifying both the expression and localization of pSTAT1. (C) Cells were treated with IFNβ for the designated durations, shown is a graph depicting nuclear p-STAT1. (D) The cells were also collected for RNA analysis and a QRT-PCR for SOCS1 expression was performed. (E) H1299<sup>175</sup> cells were introduced with RNAi against LacZ as a control or against SOCS1. p = 0.002 (F) Cells were then treated with IFNβ for 24 h. Shown is a QRT-PCR analysis of MX1 expression. p<0.05. (G) Cells were seeded in trans-wells in serum-free media and treated with IFNβ for 24 h. Migrating cells were collected and counted.</p>