10.1371/journal.pone.0061353.g004 Shalom Madar Shalom Madar Einav Harel Einav Harel Ido Goldstein Ido Goldstein Yan Stein Yan Stein Ira Kogan-Sakin Ira Kogan-Sakin Iris Kamer Iris Kamer Hilla Solomon Hilla Solomon Elya Dekel Elya Dekel Perry Tal Perry Tal Naomi Goldfinger Naomi Goldfinger Gilgi Friedlander Gilgi Friedlander Varda Rotter Varda Rotter Mutant p53 counteracts IFNβ by SOCS1-mediated attenuation of STAT1 phosphorylation. Public Library of Science 2013 genetics gene expression DNA transcription RNA stability Cancer genetics Gene networks Genetic mutation Genetics of disease immunology Immunologic subspecialties Tumor immunology Molecular cell biology Signal transduction Signaling in cellular processes Antiapoptotic signaling Signaling in selected disciplines Oncogenic signaling Clinical immunology oncology Cancers and neoplasms Lung and intrathoracic tumors Adenocarcinoma of the lung p53 counteracts socs1-mediated attenuation stat1 2013-04-22 02:10:40 Figure https://plos.figshare.com/articles/figure/_Mutant_p53_counteracts_IFN_946_by_SOCS1_mediated_attenuation_of_STAT1_phosphorylation_/687840 <p>(A) Cells were treated with IFNβ for 16 h, fixed and sorted by an “Image stream” FACS. The upper panel depicts representative images from each condition and the graph represents the mean pixel intensity of STAT1 positive cells for the entire population. (B) Same as in A, here the graph shows the similarity between p-STAT and DAPI staining, thereby quantifying both the expression and localization of pSTAT1. (C) Cells were treated with IFNβ for the designated durations, shown is a graph depicting nuclear p-STAT1. (D) The cells were also collected for RNA analysis and a QRT-PCR for SOCS1 expression was performed. (E) H1299<sup>175</sup> cells were introduced with RNAi against LacZ as a control or against SOCS1. p = 0.002 (F) Cells were then treated with IFNβ for 24 h. Shown is a QRT-PCR analysis of MX1 expression. p<0.05. (G) Cells were seeded in trans-wells in serum-free media and treated with IFNβ for 24 h. Migrating cells were collected and counted.</p>