Dataset for: Structural and functional analyses of the cellulase transcription regulator CelR
Yaoyao Fu
Soo-Jin Yeom
Kil Koang Kwon
Jungwon Hwang
Haseong Kim
Eui-jeon Woo
Dae-Hee Lee
Seung-Goo Lee
10.6084/m9.figshare.6871073.v1
https://wiley.figshare.com/articles/dataset/Dataset_for_Structural_and_functional_analyses_of_the_cellulase_transcription_regulator_CelR/6871073
CelR is a transcriptional regulator that controls the expression of cellulases catalyzing cellulose hydrolysis. However, the structural mechanism of its regulation has remained unclear. Here, we report the first structure of CelR, in this case with cellobiose bound. CelR consists of a DNA binding domain (DBD) and a regulatory domain (RD), and homodimerizes with each monomer bound to cellobiose. A hinge region (HR) in CelR connects the DBD with the RD, and Leu59 in the HR acts as a “leucine lever” that transduces a transcriptional-activation signal. Furthermore, an α4 helix mediates the ligand-binding signal for transcriptional activation. Tyr84 and Gln301 can potentially alter the ligand specificity of CelR. This study provides a pivotal step toward understanding transcription of the cellulases.
2018-08-28 08:22:59
Cellulase transcription factor
CelR
Leucine lever
Biophysics
Synthetic Biology
Biochemistry
Plant Biology
Virology
Receptors and Membrane Biology
Computational Biology
Immunology
Neuroscience
Cell Development, Proliferation and Death
Molecular Biology
Evolutionary Biology
Signal Transduction
Cancer Cell Biology
Systems Biology
Structural Biology