%0 Generic %A Redondo-Blanco, Beatriz %A Langenhan, Tobias %D 2018 %T Supplemental Material for Redondo-Blanco and Langenhan, 2018 %U https://gsajournals.figshare.com/articles/dataset/Supplemental_Material_for_Redondo-Blanco_and_Langenhan_2018/6860723 %R 10.25387/g3.6860723.v1 %2 https://ndownloader.figshare.com/files/12506531 %2 https://ndownloader.figshare.com/files/12506528 %K phic31 %K transgenesis %K Drosophila melanogaster %K CRISPR %K orthogonal %K Genomics %X

Figure S1. (A-C) Plasmid maps and information of vectors generated in this study. (D) Step-by-step protocol for the design of primers to construct a gene-specific HDR vector based on pHD-attPCC-FRT-mW-FRT.

Figure S2. (A) Location of primers and main genetic elements on doubly modified chromosome for transgene insertion. (B) Exemplary genotyping result before phiC31-mediated integration of two transgenes into the same genome (2), after integration before (3) and after (4) recombinase-assisted marker removal from both engineered loci.

%I GSA Journals