10.1021/acs.biomac.8b00588.s001
Megumu Sakoda
Megumu
Sakoda
Makoto Kaneko
Makoto
Kaneko
Seiichi Ohta
Seiichi
Ohta
Pan Qi
Pan
Qi
Shigetoshi Ichimura
Shigetoshi
Ichimura
Yutaka Yatomi
Yutaka
Yatomi
Taichi Ito
Taichi
Ito
Injectable Hemostat Composed of a Polyphosphate-Conjugated
Hyaluronan Hydrogel
American Chemical Society
2018
hydrogel
HA
HAX-PolyP
Injectable Hemostat Composed
Polyphosphate-Conjugated Hyaluronan Hydrogel
vivo
PolyP content
2018-07-09 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Injectable_Hemostat_Composed_of_a_Polyphosphate-Conjugated_Hyaluronan_Hydrogel/6847460
We
have developed a new hydrogel hemostat composed of hyaluronan
(HA) conjugated with inorganic polyphosphate (PolyP). A hemostatic
hydrogel, HAX-PolyP, was formed rapidly by mixing aldehyde-modified
HA and hydrazide-modified HA conjugated with PolyP (HA-PolyP). Although
the gelation rate decreased with increasing PolyP content, the gelation
time was below 5 min. In addition, the hydrogel swelling volume decreased
with increasing PolyP content, but the degradation rate did not depend
on PolyP content and the hydrogel underwent complete degradation through
hydrolysis over 3 weeks in phosphate buffered saline. HAX-PolyP showed
similar biocompatibility with the HA hydrogel without PolyP conjugation
in vitro and in vivo. Intraperitoneal administration of HAX-PolyP
did not induce any adhesion in the peritoneum and clot formation in
the lungs. Finally, HA-PolyP accelerated the coagulation rate of human
plasma ex vivo, and HAX-PolyP showed as strong a hemostatic effect
as fibrin glue in a mouse liver bleeding model in vivo.