10.1021/acs.biomac.8b00588.s001 Megumu Sakoda Megumu Sakoda Makoto Kaneko Makoto Kaneko Seiichi Ohta Seiichi Ohta Pan Qi Pan Qi Shigetoshi Ichimura Shigetoshi Ichimura Yutaka Yatomi Yutaka Yatomi Taichi Ito Taichi Ito Injectable Hemostat Composed of a Polyphosphate-Conjugated Hyaluronan Hydrogel American Chemical Society 2018 hydrogel HA HAX-PolyP Injectable Hemostat Composed Polyphosphate-Conjugated Hyaluronan Hydrogel vivo PolyP content 2018-07-09 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Injectable_Hemostat_Composed_of_a_Polyphosphate-Conjugated_Hyaluronan_Hydrogel/6847460 We have developed a new hydrogel hemostat composed of hyaluronan (HA) conjugated with inorganic polyphosphate (PolyP). A hemostatic hydrogel, HAX-PolyP, was formed rapidly by mixing aldehyde-modified HA and hydrazide-modified HA conjugated with PolyP (HA-PolyP). Although the gelation rate decreased with increasing PolyP content, the gelation time was below 5 min. In addition, the hydrogel swelling volume decreased with increasing PolyP content, but the degradation rate did not depend on PolyP content and the hydrogel underwent complete degradation through hydrolysis over 3 weeks in phosphate buffered saline. HAX-PolyP showed similar biocompatibility with the HA hydrogel without PolyP conjugation in vitro and in vivo. Intraperitoneal administration of HAX-PolyP did not induce any adhesion in the peritoneum and clot formation in the lungs. Finally, HA-PolyP accelerated the coagulation rate of human plasma ex vivo, and HAX-PolyP showed as strong a hemostatic effect as fibrin glue in a mouse liver bleeding model in vivo.