Geng, Jie J. Zaitouna, Anita Raghavan, Malini RIT HLA-B allotypes of the Bw4 group are more efficient in inhibiting KIR3DL1<sup>+</sup> NK cell activation in the presence of the viral TAP inhibitor BNLF2a. <p>HLA-I deficient K562 cells infected with retrovirus encoding exogenous HLA-B*44:03 and HLA-B*57:03 or retrovirus lacking HLA-B (vector) were chosen and further infected with a BNLF2a-encoding retrovirus or retrovirus lacking BNLF2a (vector). Intracellular BNLF2a expression levels (A) and cell surface expression of HLA-B were assessed by flow cytometry (B). HLA-B*44:03 expression is more strongly reduced by BNLF2a than HLA-B*57:03 (one representative experiment of three measurements is shown). (C) K562 cell based NK cell activation assay was performed with PBMCs from three different donors (D187, D136 and D215). CD3<sup>-</sup>CD56<sup>+</sup>KIR3DL1<sup>+</sup> cells were gated and NK cell activation was assessed by quantifying IFN-γ expressing population. One representative dataset from two experiments is shown.</p> peptide loading efficiency;antigen processing;Selected HLA-B allotypes;HLA-I down-modulation-induced NK cell activation;MHC-I molecules;Many viruses encode inhibitors;ER;CD 8;TAP inhibitor-induced HLA-I down-modulation;PLC;HLA-I assembly pathway;TAP 2-deficient cells;T cell responses;HLA-B allotypes;MHC-I assembly pathway 2018-07-11
    https://plos.figshare.com/articles/figure/RIT_HLA-B_allotypes_of_the_Bw4_group_are_more_efficient_in_inhibiting_KIR3DL1_sup_sup_NK_cells_activation_in_the_presence_of_the_viral_TAP_inhibitor_BNLF2a_/6806918
10.1371/journal.ppat.1007171.g006