%0 Figure %A Yeh, Wei-Lan %A Shioda, Keiko %A R. Coser, Kathryn %A Rivizzigno, Danielle %A R. McSweeney, Kristen %A Shioda, Toshi %D 2013 %T PP1 or AZD0530 tyrosine kinase inhibitors had no effect on ERα protein degradation in MCF-7 cells. %U https://plos.figshare.com/articles/figure/_PP1_or_AZD0530_tyrosine_kinase_inhibitors_had_no_effect_on_ER_945_protein_degradation_in_MCF_7_cells_/675134 %R 10.1371/journal.pone.0060889.g005 %2 https://ndownloader.figshare.com/files/1015036 %K azd0530 %K tyrosine %K kinase %K inhibitors %K had %K degradation %K mcf-7 %X

In panels (A) and (B), ERα protein expression after 6-hour exposure to 100 nM fulvestrant in the presence of PP1 or AZD0530 was determined by Western blotting (A) and ELISA (B, mean±SEM of three or more independent experiments. Asterisks indicate statistical significance, p<0.05, to fulvestrant-only group). In panels (C) and (D), fulvestrant concentration was reduced as indicated, and ERα protein expression after 6-hour exposure in the presence of PP1 or AZD0530 was determined by Western blotting (C). Panel (D) shows a typical densitometric quantitation of the ERα protein band. Three independently performed experiments did not show statistically significant effects of PP1 or AZD0530.

%I PLOS ONE