%0 Figure %A Forsman, Huamei %A Önnheim, Karin %A Andréasson, Emil %A Christenson, Karin %A Karlsson, Anna %A Bylund, Johan %A Dahlgren, Claes %D 2013 %T Receptor cross talk between neutrophil FPR1 and PAFR/CXCR1/2 determined as superoxide production. %U https://plos.figshare.com/articles/figure/_Receptor_cross_talk_between_neutrophil_FPR1_and_PAFR_CXCR1_2_determined_as_superoxide_production_/661924 %R 10.1371/journal.pone.0060169.g001 %2 https://ndownloader.figshare.com/files/1000147 %K neutrophil %K fpr1 %K superoxide %X

Human neutrophils desensitized with fMIFL were cross-desensitized to IL8 (A) but primed in their response to PAF (B). Neutrophils (105 cells, 37°C) were first activated by the FPR1 specific agonist fMIFL (0.1 nM, added at time indicated by the arrows to the left) leading to receptor desensitization (solid lines in A and B). A second stimulus (A; IL8, 100 ng/ml, B; PAF, 100 nM) was added to the cells (solid lines) at the time point indicated by the arrows to the right. Activation of naïve (non-desensitized) neutrophils by IL8 (A) and PAF (B) was determined in parallel and is shown for comparison (broken lines). A representative experiment is shown, n>5. Abscissa, time of study (min); Ordinate, superoxide production (counts per minute×106; Mcpm).

%I PLOS ONE