10.1371/journal.pone.0060169.g001 Huamei Forsman Huamei Forsman Karin Önnheim Karin Önnheim Emil Andréasson Emil Andréasson Karin Christenson Karin Christenson Anna Karlsson Anna Karlsson Johan Bylund Johan Bylund Claes Dahlgren Claes Dahlgren Receptor cross talk between neutrophil FPR1 and PAFR/CXCR1/2 determined as superoxide production. Public Library of Science 2013 neutrophil fpr1 superoxide 2013-03-29 09:43:09 Figure https://plos.figshare.com/articles/figure/_Receptor_cross_talk_between_neutrophil_FPR1_and_PAFR_CXCR1_2_determined_as_superoxide_production_/661924 <p>Human neutrophils desensitized with fMIFL were cross-desensitized to IL8 (<b>A</b>) but primed in their response to PAF (<b>B</b>). Neutrophils (10<sup>5</sup> cells, 37°C) were first activated by the FPR1 specific agonist fMIFL (0.1 nM, added at time indicated by the arrows to the left) leading to receptor desensitization (solid lines in <b>A</b> and <b>B</b>). A second stimulus (<b>A</b>; IL8, 100 ng/ml, <b>B</b>; PAF, 100 nM) was added to the cells (solid lines) at the time point indicated by the arrows to the right. Activation of naïve (non-desensitized) neutrophils by IL8 (<b>A</b>) and PAF (<b>B</b>) was determined in parallel and is shown for comparison (broken lines). A representative experiment is shown, n>5. Abscissa, time of study (min); Ordinate, superoxide production (counts per minute×10<sup>6</sup>; Mcpm).</p>