10.1371/journal.pone.0060169.g001
Huamei Forsman
Huamei
Forsman
Karin Önnheim
Karin
Önnheim
Emil Andréasson
Emil
Andréasson
Karin Christenson
Karin
Christenson
Anna Karlsson
Anna
Karlsson
Johan Bylund
Johan
Bylund
Claes Dahlgren
Claes
Dahlgren
Receptor cross talk between neutrophil FPR1 and PAFR/CXCR1/2 determined as superoxide production.
Public Library of Science
2013
neutrophil
fpr1
superoxide
2013-03-29 09:43:09
Figure
https://plos.figshare.com/articles/figure/_Receptor_cross_talk_between_neutrophil_FPR1_and_PAFR_CXCR1_2_determined_as_superoxide_production_/661924
<p>Human neutrophils desensitized with fMIFL were cross-desensitized to IL8 (<b>A</b>) but primed in their response to PAF (<b>B</b>). Neutrophils (10<sup>5</sup> cells, 37°C) were first activated by the FPR1 specific agonist fMIFL (0.1 nM, added at time indicated by the arrows to the left) leading to receptor desensitization (solid lines in <b>A</b> and <b>B</b>). A second stimulus (<b>A</b>; IL8, 100 ng/ml, <b>B</b>; PAF, 100 nM) was added to the cells (solid lines) at the time point indicated by the arrows to the right. Activation of naïve (non-desensitized) neutrophils by IL8 (<b>A</b>) and PAF (<b>B</b>) was determined in parallel and is shown for comparison (broken lines). A representative experiment is shown, n>5. Abscissa, time of study (min); Ordinate, superoxide production (counts per minute×10<sup>6</sup>; Mcpm).</p>