%0 Figure %A Kratchmarov, Radomir %A P. Taylor, Matthew %A W. Enquist, Lynn %D 2013 %T Subcellular localization of GFP-Us9 and phospho-GFP-Us9 in axons. %U https://plos.figshare.com/articles/figure/_Subcellular_localization_of_GFP_Us9_and_phospho_GFP_Us9_in_axons_/655098 %R 10.1371/journal.pone.0058776.g002 %2 https://ndownloader.figshare.com/files/991065 %K localization %K gfp-us9 %K phospho-gfp-us9 %X

A.) Representative IF visualization of total GFP-Us9 in axonal vesicles and phosphorylated Us9 subspecies detected by phospho-specific mouse monoclonal 2D5E6 at 8 hours post-infection with PRV 340. Discrete puncta are highlighted: arrows indicate dual positive GFP-Us9 (+)/Alexafluor 568 (+) structures, triangles denote single positive GFP-Us9 (+) structures. B.) Quantitation of phosphorylation status of Us9 puncta in axons. A total of 626 GFP-Us9 puncta were scored in axons and the percentage phosphorylated determined through IF. GFP-Us9 puncta were counted across 3 biological replicates.

%I PLOS ONE