10.1371/journal.pone.0058857.g007 Aseervatham Anusha Amali Aseervatham Anusha Amali Lawrence Sie Lawrence Sie Christoph Winkler Christoph Winkler Mark Featherstone Mark Featherstone <i>scl1</i> and <i>lmo2</i> act downstream of <i>hoxd4a</i> to direct formation of the hemangioblast. Public Library of Science 2013 findings place hoxd 4a cdx Hox genes ventral-most mesoderm fated hoxd 4a function group paralog Hoxd 4 display malformations 13 hours post fertilization meis 1.1 expression hemangioblast markers scl 1 hoxd 4a acts PLM orthologous hoxd 4a gene Zebrafish hoxd 4a Acts Upstream meis 1.1 2013-03-16 07:36:56 Figure https://plos.figshare.com/articles/figure/_scl1_and_lmo2_act_downstream_of_hoxd4a_to_direct_formation_of_the_hemangioblast_/653062 <p>All images are of <i>hoxd4a</i> morphants at 13 hpf. <i>In situ</i> hybridization was performed to detect expression of <i>scl1</i> and <i>lmo2</i> (A–D), <i>gata1</i> and <i>hbbe1</i> (E–H) and <i>fli1</i> and <i>flk1</i> (I–L). To test for rescue of gene expression, embryos were co-injected with capped mRNA for either <i>scl1</i> or <i>fli1</i> as indicated on the left. Ratios indicate the fraction of embryos showing the presented phenotype. Scale bars equal 100 µm.</p>