10.6084/m9.figshare.6505910.v1 Hong Chen Hong Chen Hai-Xiang Shen Hai-Xiang Shen Yi-Wei Lin Yi-Wei Lin Ye-Qing Mao Ye-Qing Mao Ben Liu Ben Liu Li-Ping Xie Li-Ping Xie Small RNA-induced INTS6 gene up-regulation suppresses castration-resistant prostate cancer cells by regulating β-catenin signaling Taylor & Francis Group 2018 RNA activation INTS6 castration-resistant prostate cancer 2018-06-13 05:57:37 Dataset https://tandf.figshare.com/articles/dataset/Small_RNA-induced_INTS6_gene_up-regulation_suppresses_castration-resistant_prostate_cancer_cells_by_regulating_-catenin_signaling/6505910 <p>Small RNAs play an important role in gene regulatory networks. The gene suppressive effect of small RNAs was previously the dominant focus of studies, but during the recent decade, small RNA-induced gene activation has been reported and has become a notable gene manipulation technique. In this study, a putative tumor suppressor, <i>INTS6</i>, was activated by introducing a promoter-targeted small RNA (dsRNA-915) into castration-resistant prostate cancer (CRPC) cells. Unique dynamics associated with the gene upregulation phenomenon was observed. Following gene activation, cell proliferation and motility were suppressed in vitro. Downregulation of <i>Wnt</i>/β-catenin signaling was observed during the activation period, and the impairment of β-catenin degradation reversed the tumor suppressor effects of <i>INTS6</i>. These results suggest the potential application of small activating RNAs in targeted gene therapy for CRPC.</p>