10.6084/m9.figshare.6505910.v1
Hong Chen
Hong
Chen
Hai-Xiang Shen
Hai-Xiang
Shen
Yi-Wei Lin
Yi-Wei
Lin
Ye-Qing Mao
Ye-Qing
Mao
Ben Liu
Ben
Liu
Li-Ping Xie
Li-Ping
Xie
Small RNA-induced INTS6 gene up-regulation suppresses castration-resistant prostate cancer cells by regulating β-catenin signaling
Taylor & Francis Group
2018
RNA activation
INTS6
castration-resistant prostate cancer
2018-06-13 05:57:37
Dataset
https://tandf.figshare.com/articles/dataset/Small_RNA-induced_INTS6_gene_up-regulation_suppresses_castration-resistant_prostate_cancer_cells_by_regulating_-catenin_signaling/6505910
<p>Small RNAs play an important role in gene regulatory networks. The gene suppressive effect of small RNAs was previously the dominant focus of studies, but during the recent decade, small RNA-induced gene activation has been reported and has become a notable gene manipulation technique. In this study, a putative tumor suppressor, <i>INTS6</i>, was activated by introducing a promoter-targeted small RNA (dsRNA-915) into castration-resistant prostate cancer (CRPC) cells. Unique dynamics associated with the gene upregulation phenomenon was observed. Following gene activation, cell proliferation and motility were suppressed in vitro. Downregulation of <i>Wnt</i>/β-catenin signaling was observed during the activation period, and the impairment of β-catenin degradation reversed the tumor suppressor effects of <i>INTS6</i>. These results suggest the potential application of small activating RNAs in targeted gene therapy for CRPC.</p>