%0 Journal Article %A Ma, Chengxian %A Ha, Kyungsoo %A Kim, Min-su %A Noh, Young-Woock %A Lin, Han %A Tang, Lichun %A Zhu, Qing %A Zhang, Dan %A Chen, Huan %A Han, Suxia %A Zhang, Pumin %D 2018 %T The anaphase promoting complex promotes NHEJ repair through stabilizing Ku80 at DNA damage sites %U https://tandf.figshare.com/articles/journal_contribution/The_anaphase_promoting_complex_promotes_NHEJ_repair_through_stabilizing_Ku80_at_DNA_damage_sites/6505601 %R 10.6084/m9.figshare.6505601.v1 %2 https://ndownloader.figshare.com/files/11965043 %K Anaphase promoting complex %K Cdh1 %K double-strand breaks %K RNF8 %K NHEJ repair %X

Double-strand breaks (DSBs) are repaired through two major pathways, homology-directed recombination (HDR) and non-homologous end joining (NHEJ). The choice between these two pathways is largely influenced by cell cycle phases. HDR can occur only in S/G2 when sister chromatid can provide homologous templates, whereas NHEJ can take place in all phases of the cell cycle except mitosis. Central to NHEJ repair is the Ku70/80 heterodimer which forms a ring structure that binds DSB ends and serves as a platform to recruit factors involved in NHEJ. Upon completion of NHEJ repair, DNA double strand-encircling Ku dimers have to be removed. The removal depends on ubiquitylation and proteasomal degradation of Ku80 by the ubiquitin E3 ligases RNF8. Here we report that RNF8 is a substrate of APCCdh1 and the latter keeps RNF8 level in check at DSBs to prevent premature turnover of Ku80.

%I Taylor & Francis