10.6084/m9.figshare.6505418.v1 Nazanin Asghari Hanjani Nazanin Asghari Hanjani Negar Zamaninour Negar Zamaninour Narjes Najibi Narjes Najibi Agha Fatemeh Hosseini Agha Fatemeh Hosseini Tayebeh Rastegar Tayebeh Rastegar Mohammad Reza Vafa Mohammad Reza Vafa The role of time of food intake on upcoming liver disease in male Wistar rat Taylor & Francis Group 2018 NAFLD calorie restriction circadian food intake time-restricted feeding juvenile obesity visceral fat HFD: high-fat diet CON1: first-phase control group CR: calorie restriction NIF: night intermittent fasting DIF: day intermittent fasting CON2: second-phase control group SCN: suprachiasmatic nucleus ZT: zeitgeber ANONA ALT: alanine aminotransferase AST: aspartate aminotransferase ANOVA: one-way analysis of variance 2018-06-13 04:21:18 Journal contribution https://tandf.figshare.com/articles/journal_contribution/The_role_of_time_of_food_intake_on_upcoming_liver_disease_in_male_Wistar_rat/6505418 <p>Interventions against obesity, are mainly around changing calorie intake and energy expenditure. Recently, some studies focused on the influence of circadian time of food intake on metabolic status. Here, we compare the role of calorie restriction and time restricted feeding followed by high-fat diet started post weaning, First, 52 male Wistarrats (3 weeks old) were divided into two groups: the high-fat diet (HFD, <i>n</i> = 42) and the control group (CON1, <i>n</i> = 11). After 17 weeks, five rats were randomly selected from each group for sample preparation. In the second phase, the animals in HFD group were assigned into four groups (<i>n</i> = 9): (1) 30% calorie restriction (CR), (2) day intermittent fasting (DIF), (3) night intermittent fasting (NIF), (4) adlibitum food intake (AL), (5) remained animal from the first phase control (CON2). Seventeen weeks of HFD started post-weaning did not cause fatty liver but it caused a significant difference in the body and the adipose tissue weight (P0.05). The results showed that longtime HFD did not lead to liver steatosis while the incorrect time of food intake predisposes the animal to the upcoming liver disease. This data indicate a significant role of timing of food intake rather than nutrition composition itself.</p>